Electron Diffraction from a Low Temperature Oriented Molecular Beam

John Spence (Inventor)

Research output: Patent

Abstract

This invention is an apparatus which generates a beam of hydrated molecules at the temperature of liquid helium within a vacuum chamber. This beam passes through a laser beam, which rotates all the molecules into the same orientation. The beam then passes across a coherent electron beam which produces an electron diffraction pattern. The use of the apparatus is to solve the structure, at atomic resolution, of proteins and other molecules which do not readily form the crystals needed for X-ray crystallography. The structures are solved using these electron diffraction patterns and new iterative solutions to the phase problem, together with the heavy-atom method. Charge-density maps are obtained for many orientations of the molecule, and synthesized tomographically to generate a three-dimensional view.
Original languageEnglish (US)
StatePublished - Feb 16 2004

Fingerprint

molecular beams
electron diffraction
molecules
diffraction patterns
iterative solution
inventions
vacuum chambers
liquid helium
crystallography
laser beams
electron beams
proteins
crystals
atoms
x rays
temperature

Cite this

@misc{08a2adf9363c4cecb2a3431c3b02a41b,
title = "Electron Diffraction from a Low Temperature Oriented Molecular Beam",
abstract = "This invention is an apparatus which generates a beam of hydrated molecules at the temperature of liquid helium within a vacuum chamber. This beam passes through a laser beam, which rotates all the molecules into the same orientation. The beam then passes across a coherent electron beam which produces an electron diffraction pattern. The use of the apparatus is to solve the structure, at atomic resolution, of proteins and other molecules which do not readily form the crystals needed for X-ray crystallography. The structures are solved using these electron diffraction patterns and new iterative solutions to the phase problem, together with the heavy-atom method. Charge-density maps are obtained for many orientations of the molecule, and synthesized tomographically to generate a three-dimensional view.",
author = "John Spence",
year = "2004",
month = "2",
day = "16",
language = "English (US)",
type = "Patent",

}

TY - PAT

T1 - Electron Diffraction from a Low Temperature Oriented Molecular Beam

AU - Spence, John

PY - 2004/2/16

Y1 - 2004/2/16

N2 - This invention is an apparatus which generates a beam of hydrated molecules at the temperature of liquid helium within a vacuum chamber. This beam passes through a laser beam, which rotates all the molecules into the same orientation. The beam then passes across a coherent electron beam which produces an electron diffraction pattern. The use of the apparatus is to solve the structure, at atomic resolution, of proteins and other molecules which do not readily form the crystals needed for X-ray crystallography. The structures are solved using these electron diffraction patterns and new iterative solutions to the phase problem, together with the heavy-atom method. Charge-density maps are obtained for many orientations of the molecule, and synthesized tomographically to generate a three-dimensional view.

AB - This invention is an apparatus which generates a beam of hydrated molecules at the temperature of liquid helium within a vacuum chamber. This beam passes through a laser beam, which rotates all the molecules into the same orientation. The beam then passes across a coherent electron beam which produces an electron diffraction pattern. The use of the apparatus is to solve the structure, at atomic resolution, of proteins and other molecules which do not readily form the crystals needed for X-ray crystallography. The structures are solved using these electron diffraction patterns and new iterative solutions to the phase problem, together with the heavy-atom method. Charge-density maps are obtained for many orientations of the molecule, and synthesized tomographically to generate a three-dimensional view.

M3 - Patent

ER -