TY - JOUR
T1 - Electron-acceptor loadings affect chloroform dechlorination in a hydrogen-based membrane biofilm reactor
AU - Lai, Yen Jung Sean
AU - Ontiveros-Valencia, Aura
AU - Coskun, Tamer
AU - Zhou, Chen
AU - Rittmann, Bruce
N1 - Funding Information:
Support for this research was provided by an endowment from Brian Swette and Family. We appreciate the provision of Zara-10 cultures by Drs. Krajmalnik-Brown and Delgado from the School of Sustainable Engineering and the Built Environment at Arizona State University.
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
PY - 2019/6
Y1 - 2019/6
N2 - Chloroform (CF) can undergo reductive dechlorination to dichloromethane, chloromethane, and methane. However, competition for hydrogen (H 2 ), the electron-donor substrate, may cause poor dechlorination when multiple electron acceptors are present. Common acceptors in anaerobic environments are nitrate (NO 3 − ), sulfate (SO 4 2− ), and bicarbonate (HCO 3 − ). We evaluated CF dechlorination in the presence of HCO 3 − at 1.56 e − Eq/m 2 -day, then NO 3 − at 0.04–0.15 e − Eq/m 2 -day, and finally NO 3 − (0.04 e − Eq/m 2 -day) along with SO 4 2− at 0.33 e − Eq/m 2 -day in an H 2 -based membrane biofilm reactor (MBfR). When the biofilm was initiated with CF-dechlorination conditions (no NO 3 − or SO 4 2− ), it yielded a CF flux of 0.14 e − Eq/m 2 -day and acetate production via homoacetogenesis up to 0.26 e − eq/m 2 -day. Subsequent addition of NO 3 − at 0.05 e − Eq/m 2 -day maintained full CF dechlorination and homoacetogenesis, but NO 3 − input at 0.15 e − Eq/m 2 -day caused CF to remain in the reactor's effluent and led to negligible acetate production. The addition of SO 4 2− did not affect CF reduction, but SO 4 2− reduction significantly altered the microbial community by introducing sulfate-reducing Desulfovibrio and more sulfur-oxidizing Arcobacter. Dechloromonas appeared to carry out CF dechlorination and denitrification, whereas Acetobacterium (homoacetogen) may have been involved with hydrolytic dechlorination. Modifications to the electron acceptors fed to the MBfR caused the microbial community to undergo changes in structure that reflected changes in the removal fluxes.
AB - Chloroform (CF) can undergo reductive dechlorination to dichloromethane, chloromethane, and methane. However, competition for hydrogen (H 2 ), the electron-donor substrate, may cause poor dechlorination when multiple electron acceptors are present. Common acceptors in anaerobic environments are nitrate (NO 3 − ), sulfate (SO 4 2− ), and bicarbonate (HCO 3 − ). We evaluated CF dechlorination in the presence of HCO 3 − at 1.56 e − Eq/m 2 -day, then NO 3 − at 0.04–0.15 e − Eq/m 2 -day, and finally NO 3 − (0.04 e − Eq/m 2 -day) along with SO 4 2− at 0.33 e − Eq/m 2 -day in an H 2 -based membrane biofilm reactor (MBfR). When the biofilm was initiated with CF-dechlorination conditions (no NO 3 − or SO 4 2− ), it yielded a CF flux of 0.14 e − Eq/m 2 -day and acetate production via homoacetogenesis up to 0.26 e − eq/m 2 -day. Subsequent addition of NO 3 − at 0.05 e − Eq/m 2 -day maintained full CF dechlorination and homoacetogenesis, but NO 3 − input at 0.15 e − Eq/m 2 -day caused CF to remain in the reactor's effluent and led to negligible acetate production. The addition of SO 4 2− did not affect CF reduction, but SO 4 2− reduction significantly altered the microbial community by introducing sulfate-reducing Desulfovibrio and more sulfur-oxidizing Arcobacter. Dechloromonas appeared to carry out CF dechlorination and denitrification, whereas Acetobacterium (homoacetogen) may have been involved with hydrolytic dechlorination. Modifications to the electron acceptors fed to the MBfR caused the microbial community to undergo changes in structure that reflected changes in the removal fluxes.
KW - Dechloromonas
KW - dechlorination
KW - electron-acceptor competition
KW - membrane biofilm
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U2 - 10.1002/bit.26945
DO - 10.1002/bit.26945
M3 - Article
C2 - 30712264
AN - SCOPUS:85062358274
SN - 0006-3592
VL - 116
SP - 1439
EP - 1448
JO - Biotechnology and bioengineering
JF - Biotechnology and bioengineering
IS - 6
ER -