Electro-selective fermentation enhances lipid extraction and biohydrogenation of Scenedesmus acutus biomass

Yuanzhe Liu, Yen Jung Sean Lai, Thiago Stangherlin Barbosa, Rashmi Chandra, Prathap Parameswaran, Bruce Rittmann

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Extraction of lipids from microalgae has been economically and energy prohibitive due to the requirement for pretreatment, and the use of toxic solvents in the process has exacerbated the problem. Selective Fermentation (SF) helps overcome these problems by selectively biodegrading carbohydrate and protein, while conserving lipids. We introduced and evaluated electro-selective fermentation (ESF), in which anode respiration in a microbial electrolysis cell (MEC) altered fermentation performance in ways that enhanced lipid wet-extraction. Using Scenedesmus acutus biomass, we evaluated ESF and compared it to SF. Compared to SF, ESF increased protein degradation by 4-fold, even though anode respiration represented <1% of the total electron input. Although ESF led to greater loss of total lipids, it improved lipid wet-extraction efficiency using a non-toxic solvent by 3-fold. Lipid loss was due to β-oxidation linked to biohydrogenation, and the long-chain fatty acid (LCFA) profile shifted from C18:1 to C16:0 and C14:0. Microbial community analysis emphasized the presence of protein-degrading bacteria and biohydrogenators in the ESF suspension and anode-respiring bacteria (ARB) on the ESF anode. Overall, ESF improved lipid extractability and biofuel quality despite current being a small component in the overall COD balance.

Original languageEnglish (US)
Article number101397
JournalAlgal Research
Volume38
DOIs
StatePublished - Mar 1 2019
Externally publishedYes

Fingerprint

biohydrogenation
Scenedesmus
fermentation
biomass
lipids
cell respiration
bacteria
long chain fatty acids
protein degradation
biofuels
microalgae
microbial communities
proteins
pretreatment
fatty acid composition
electrons

Keywords

  • Biohydrogenation
  • Electro-selective fermentation (ESF)
  • Lipids
  • Long-chain fatty acids (LCFA)
  • Microbial electrolysis cells (MEC)
  • Scenedesmus

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Electro-selective fermentation enhances lipid extraction and biohydrogenation of Scenedesmus acutus biomass. / Liu, Yuanzhe; Lai, Yen Jung Sean; Barbosa, Thiago Stangherlin; Chandra, Rashmi; Parameswaran, Prathap; Rittmann, Bruce.

In: Algal Research, Vol. 38, 101397, 01.03.2019.

Research output: Contribution to journalArticle

Liu, Yuanzhe ; Lai, Yen Jung Sean ; Barbosa, Thiago Stangherlin ; Chandra, Rashmi ; Parameswaran, Prathap ; Rittmann, Bruce. / Electro-selective fermentation enhances lipid extraction and biohydrogenation of Scenedesmus acutus biomass. In: Algal Research. 2019 ; Vol. 38.
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