Electric triggering for enhanced control of droplet generation

Daihyun Kim, Austin Echelmeier, Jorvani Cruz Villarreal, Sahir Gandhi, Sebastian Quintana, Ana Egatz-Gomez, Alexandra Ros

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Serial femtosecond crystallography (SFX) is a powerful technique that uses X-ray free-electron lasers (XFEL) to determine structures of biomolecular complexes. Specifically, it benefits the study of atomic resolution structures of large membrane protein complexes and time-resolved reactions with crystallography. One major drawback of SFX studies with XFELs is the consumption of large amounts of a protein crystal sample to collect a complete X-ray diffraction data set for high-resolution crystal structures. This increases the time and resources required for sample preparation and experimentation. The intrinsic pulsed nature of all current X-ray sources is a major reason why such large amounts of sample are required. Any crystal sample that is delivered in the path of the X-ray beam during its "off-time" is wasted. To address this large sample consumption issue, we developed a 3D printed microfluidic system with integrated metal electrodes for water-in-oil droplet generation to dynamically create and manipulate aqueous droplets. We demonstrate on-demand droplet generation using DC potentials and the ability to tune the frequency of droplet generation through the application of AC potentials. More importantly, to assist with the synchronization of droplets and XFEL pulses, we show that the device can induce a phase shift in the base droplet generation frequency. This novel approach to droplet generation has the potential to reduce sample waste by more than 95% for SFX experiments with XFELs performed with liquid jets and can operate under low- and high-pressure liquid injection systems.

Original languageEnglish (US)
Pages (from-to)9792-9799
Number of pages8
JournalAnalytical chemistry
Volume91
Issue number15
DOIs
StatePublished - Aug 6 2019

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X ray lasers
Crystallography
Free electron lasers
X rays
Crystals
Liquids
Microfluidics
Phase shift
Laser pulses
Synchronization
Oils
Membrane Proteins
Crystal structure
Metals
X ray diffraction
Electrodes
Water
Proteins
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kim, D., Echelmeier, A., Cruz Villarreal, J., Gandhi, S., Quintana, S., Egatz-Gomez, A., & Ros, A. (2019). Electric triggering for enhanced control of droplet generation. Analytical chemistry, 91(15), 9792-9799. https://doi.org/10.1021/acs.analchem.9b01449

Electric triggering for enhanced control of droplet generation. / Kim, Daihyun; Echelmeier, Austin; Cruz Villarreal, Jorvani; Gandhi, Sahir; Quintana, Sebastian; Egatz-Gomez, Ana; Ros, Alexandra.

In: Analytical chemistry, Vol. 91, No. 15, 06.08.2019, p. 9792-9799.

Research output: Contribution to journalArticle

Kim, D, Echelmeier, A, Cruz Villarreal, J, Gandhi, S, Quintana, S, Egatz-Gomez, A & Ros, A 2019, 'Electric triggering for enhanced control of droplet generation', Analytical chemistry, vol. 91, no. 15, pp. 9792-9799. https://doi.org/10.1021/acs.analchem.9b01449
Kim D, Echelmeier A, Cruz Villarreal J, Gandhi S, Quintana S, Egatz-Gomez A et al. Electric triggering for enhanced control of droplet generation. Analytical chemistry. 2019 Aug 6;91(15):9792-9799. https://doi.org/10.1021/acs.analchem.9b01449
Kim, Daihyun ; Echelmeier, Austin ; Cruz Villarreal, Jorvani ; Gandhi, Sahir ; Quintana, Sebastian ; Egatz-Gomez, Ana ; Ros, Alexandra. / Electric triggering for enhanced control of droplet generation. In: Analytical chemistry. 2019 ; Vol. 91, No. 15. pp. 9792-9799.
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