TY - JOUR
T1 - Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor
AU - Sheng, Jie
AU - Kim, Hyun Woo
AU - Badalamenti, Jonathan P.
AU - Zhou, Chao
AU - Sridharakrishnan, Swathi
AU - Krajmalnik-Brown, Rosa
AU - Rittmann, Bruce
AU - Vannela, Raveender
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.
AB - Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.
KW - Biomass production
KW - Fatty acids
KW - Lipids
KW - Synechocystis sp. PCC6803
KW - Temperature
UR - http://www.scopus.com/inward/record.url?scp=81555201029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=81555201029&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2011.09.083
DO - 10.1016/j.biortech.2011.09.083
M3 - Article
C2 - 22001056
AN - SCOPUS:81555201029
VL - 102
SP - 11218
EP - 11225
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
IS - 24
ER -