Abstract

Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.

Original languageEnglish (US)
Pages (from-to)11218-11225
Number of pages8
JournalBioresource Technology
Volume102
Issue number24
DOIs
StatePublished - Dec 2011

Fingerprint

Photobioreactors
Lipids
lipid
temperature
Temperature
Biomass
stress change
biomass
biofuel
Biofuels
effect
fatty acid
Fatty acids
Feedstocks
Nutrients
Fatty Acids
rate
nutrient

Keywords

  • Biomass production
  • Fatty acids
  • Lipids
  • Synechocystis sp. PCC6803
  • Temperature

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor. / Sheng, Jie; Kim, Hyun Woo; Badalamenti, Jonathan P.; Zhou, Chao; Sridharakrishnan, Swathi; Krajmalnik-Brown, Rosa; Rittmann, Bruce; Vannela, Raveender.

In: Bioresource Technology, Vol. 102, No. 24, 12.2011, p. 11218-11225.

Research output: Contribution to journalArticle

Sheng, Jie ; Kim, Hyun Woo ; Badalamenti, Jonathan P. ; Zhou, Chao ; Sridharakrishnan, Swathi ; Krajmalnik-Brown, Rosa ; Rittmann, Bruce ; Vannela, Raveender. / Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor. In: Bioresource Technology. 2011 ; Vol. 102, No. 24. pp. 11218-11225.
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