Abstract
Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11218-11225 |
| Number of pages | 8 |
| Journal | Bioresource Technology |
| Volume | 102 |
| Issue number | 24 |
| DOIs | |
| State | Published - Dec 2011 |
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Keywords
- Biomass production
- Fatty acids
- Lipids
- Synechocystis sp. PCC6803
- Temperature
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Waste Management and Disposal
Cite this
Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor. / Sheng, Jie; Kim, Hyun Woo; Badalamenti, Jonathan P.; Zhou, Chao; Sridharakrishnan, Swathi; Krajmalnik-Brown, Rosa; Rittmann, Bruce; Vannela, Raveender.
In: Bioresource Technology, Vol. 102, No. 24, 12.2011, p. 11218-11225.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor
AU - Sheng, Jie
AU - Kim, Hyun Woo
AU - Badalamenti, Jonathan P.
AU - Zhou, Chao
AU - Sridharakrishnan, Swathi
AU - Krajmalnik-Brown, Rosa
AU - Rittmann, Bruce
AU - Vannela, Raveender
PY - 2011/12
Y1 - 2011/12
N2 - Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.
AB - Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.
KW - Biomass production
KW - Fatty acids
KW - Lipids
KW - Synechocystis sp. PCC6803
KW - Temperature
UR - http://www.scopus.com/inward/record.url?scp=81555201029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=81555201029&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2011.09.083
DO - 10.1016/j.biortech.2011.09.083
M3 - Article
C2 - 22001056
AN - SCOPUS:81555201029
VL - 102
SP - 11218
EP - 11225
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
IS - 24
ER -