Abstract

To investigate interactions among multiple electron acceptors in a H 2-fed biofilm, we operated a membrane biofilm reactor with H 2-delivery capacity sufficient to reduce all acceptors. ClO 4 - and O2 were input electron acceptors in all stages at surface loadings of 0.08 ± 0.006 g/m2-d (1.0 ± 0.7 e- meq/m2-d) for ClO4 - and 0.51 g/m2-d (76 e- meq/m2-d) for O 2. SO4 2- was added in Stage 2 at 3.77 ± 0.39 g/m2-d (331 ± 34 e- meq/m2-d), and NO3 - was further added in Stage 3 at 0.72 ± 0.03 g N/m2-d (312 ± 13 e- meq/m2-d). At steady state for each stage, ClO4 -, O2, and NO 3 - (when present in the influent) were completely reduced; measured SO4 2- reduction decreased from 78 ± 4% in Stage 2 to 59 ± 4% in Stage 3, when NO3 - was present. While perchlorate-reducing bacteria (PRB), assayed by qPCR targeting the pcrA gene, remained stable throughout, sulfate-reducing bacteria (SRB), assayed by the dsrA gene, increased almost 3 orders of magnitude when significant SO4 2- reduction occurred in stage 2. The abundance of denitrifying bacteria (DB), assayed by the nirK and nirS genes, increased in Stage 3, while SRB remained at high numbers, but did not increase. Based on pyrosequencing analyses, β-Proteobacteria dominated in Stage 1, but ε-Proteobacteria became more important in Stages 2 and 3, when the input of multiple electron acceptors favored genera with broader electron-accepting capabilities. Sulfuricurvum (a sulfur oxidizer and NO 3 - reducer) and Desulfovibrio (a SO4 2- reducer) become dominant in Stage 3, suggesting redox cycling of sulfur in the biofilm.

Original languageEnglish (US)
Pages (from-to)7396-7403
Number of pages8
JournalEnvironmental Science and Technology
Volume47
Issue number13
DOIs
StatePublished - Jul 2 2013

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Biofilms
biofilm
Hydrogen
Bacteria
hydrogen
electron
Electrons
Genes
sulfate-reducing bacterium
Sulfur
Sulfates
gene
sulfur
bacterium
perchlorate
targeting
membrane
Membranes
effect
Proteobacteria

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Effects of multiple electron acceptors on microbial interactions in a hydrogen-based biofilm. / Zhao, He Ping; Ilhan, Zehra Esra; Ontiveros-Valencia, Aura; Tang, Youneng; Rittmann, Bruce; Krajmalnik-Brown, Rosa.

In: Environmental Science and Technology, Vol. 47, No. 13, 02.07.2013, p. 7396-7403.

Research output: Contribution to journalArticle

Zhao, He Ping ; Ilhan, Zehra Esra ; Ontiveros-Valencia, Aura ; Tang, Youneng ; Rittmann, Bruce ; Krajmalnik-Brown, Rosa. / Effects of multiple electron acceptors on microbial interactions in a hydrogen-based biofilm. In: Environmental Science and Technology. 2013 ; Vol. 47, No. 13. pp. 7396-7403.
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abstract = "To investigate interactions among multiple electron acceptors in a H 2-fed biofilm, we operated a membrane biofilm reactor with H 2-delivery capacity sufficient to reduce all acceptors. ClO 4 - and O2 were input electron acceptors in all stages at surface loadings of 0.08 ± 0.006 g/m2-d (1.0 ± 0.7 e- meq/m2-d) for ClO4 - and 0.51 g/m2-d (76 e- meq/m2-d) for O 2. SO4 2- was added in Stage 2 at 3.77 ± 0.39 g/m2-d (331 ± 34 e- meq/m2-d), and NO3 - was further added in Stage 3 at 0.72 ± 0.03 g N/m2-d (312 ± 13 e- meq/m2-d). At steady state for each stage, ClO4 -, O2, and NO 3 - (when present in the influent) were completely reduced; measured SO4 2- reduction decreased from 78 ± 4{\%} in Stage 2 to 59 ± 4{\%} in Stage 3, when NO3 - was present. While perchlorate-reducing bacteria (PRB), assayed by qPCR targeting the pcrA gene, remained stable throughout, sulfate-reducing bacteria (SRB), assayed by the dsrA gene, increased almost 3 orders of magnitude when significant SO4 2- reduction occurred in stage 2. The abundance of denitrifying bacteria (DB), assayed by the nirK and nirS genes, increased in Stage 3, while SRB remained at high numbers, but did not increase. Based on pyrosequencing analyses, β-Proteobacteria dominated in Stage 1, but ε-Proteobacteria became more important in Stages 2 and 3, when the input of multiple electron acceptors favored genera with broader electron-accepting capabilities. Sulfuricurvum (a sulfur oxidizer and NO 3 - reducer) and Desulfovibrio (a SO4 2- reducer) become dominant in Stage 3, suggesting redox cycling of sulfur in the biofilm.",
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AU - Zhao, He Ping

AU - Ilhan, Zehra Esra

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AU - Rittmann, Bruce

AU - Krajmalnik-Brown, Rosa

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