26 Citations (Scopus)

Abstract

We studied the effects of light intensity (LI) and CO2 supply on pH and total lipid production and fatty acids by Synechocystis sp. PCC6803 during continuous-flow operation of a photobioreactor having continuous nutrient supply. The temperature was fixed at 30 °C, and the LI pattern mimicked a day/night light cycle from 0 to 1920 μmol/m2 s. The CO2 supply varied from1 to 5% v/v of total air. The total lipid content increased proportionally to LI, reaching a high content of 14% of dry weight (DW) at the highest LI at 3% CO2. In contrast, LI had no significant influence on the total fatty acid content, which was 3.4% ± 0.5% DW, measured as fatty acid methyl esters (FAMEs). Palmitic acid (C16:0) was the main fatty acid (52% of FAMEs), but γ-linolenic acid (C18:3n6) and linoleic acid (C18:2) were significant at 20% and 14% of total FAMEs, respectively. Also, a-linolenic acid (C18:3n3), oleic acid (C18:1), and palmitoleic acid (C16:1) represented 5%, 4%, and 4% of the total FAMEs, respectively. In case of C16:0, its highest content was achieved at LI of 400 to 1500 μmol/m2 s and pH media values from 7.2 to 8.8 (3% CO2). The highest formation of C16:1 and C18:1 (desirable for biodiesel production) occurred with LI up to 600 μmol/m2 s at pH 9 (3% CO2). Stearic acid (C18:0) and linoleic acid (C18:2) contents did not vary with LI or pH, but α-linolenic acid (C18:3n3) formation occurred with patterns opposite to C18:3n6, C16:0, and C16:1. LI of 400 to 1600 μmol/m2 s and pH range from 7.7 to 8.7 led to the highest values of C18:3n6 (0.8% DW), but C18:3n3 was suppressed by these conditions, supporting a desaturation pathway in Synechocystis. These results point to strategies to optimize LI, CO2, and pH, to enhance the fatty acid production profile for biofuel production.

Original languageEnglish (US)
Pages (from-to)10-16
Number of pages7
JournalAlgal Research
Volume12
DOIs
StatePublished - Nov 1 2015

Fingerprint

Synechocystis
light intensity
carbon dioxide
fatty acids
lipids
linolenic acid
linoleic acid
palmitoleic acid
biodiesel
stearic acid
biofuels
palmitic acid
oleic acid
photoperiod
fatty acid composition
lipid content
air

Keywords

  • CO
  • Fatty acids
  • Light intensity
  • Lipids
  • pH
  • Synechocystis

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Effects of light intensity and carbon dioxide on lipids and fatty acids produced by Synechocystis sp. PCC6803 during continuous flow. / Cuellar-Bermudez, Sara P.; Romero-Ogawa, Miguel A.; Vannela, Raveender; Lai, YenJung Sean; Rittmann, Bruce; Parra-Saldivar, Roberto.

In: Algal Research, Vol. 12, 01.11.2015, p. 10-16.

Research output: Contribution to journalArticle

Cuellar-Bermudez, Sara P. ; Romero-Ogawa, Miguel A. ; Vannela, Raveender ; Lai, YenJung Sean ; Rittmann, Bruce ; Parra-Saldivar, Roberto. / Effects of light intensity and carbon dioxide on lipids and fatty acids produced by Synechocystis sp. PCC6803 during continuous flow. In: Algal Research. 2015 ; Vol. 12. pp. 10-16.
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abstract = "We studied the effects of light intensity (LI) and CO2 supply on pH and total lipid production and fatty acids by Synechocystis sp. PCC6803 during continuous-flow operation of a photobioreactor having continuous nutrient supply. The temperature was fixed at 30 °C, and the LI pattern mimicked a day/night light cycle from 0 to 1920 μmol/m2 s. The CO2 supply varied from1 to 5{\%} v/v of total air. The total lipid content increased proportionally to LI, reaching a high content of 14{\%} of dry weight (DW) at the highest LI at 3{\%} CO2. In contrast, LI had no significant influence on the total fatty acid content, which was 3.4{\%} ± 0.5{\%} DW, measured as fatty acid methyl esters (FAMEs). Palmitic acid (C16:0) was the main fatty acid (52{\%} of FAMEs), but γ-linolenic acid (C18:3n6) and linoleic acid (C18:2) were significant at 20{\%} and 14{\%} of total FAMEs, respectively. Also, a-linolenic acid (C18:3n3), oleic acid (C18:1), and palmitoleic acid (C16:1) represented 5{\%}, 4{\%}, and 4{\%} of the total FAMEs, respectively. In case of C16:0, its highest content was achieved at LI of 400 to 1500 μmol/m2 s and pH media values from 7.2 to 8.8 (3{\%} CO2). The highest formation of C16:1 and C18:1 (desirable for biodiesel production) occurred with LI up to 600 μmol/m2 s at pH 9 (3{\%} CO2). Stearic acid (C18:0) and linoleic acid (C18:2) contents did not vary with LI or pH, but α-linolenic acid (C18:3n3) formation occurred with patterns opposite to C18:3n6, C16:0, and C16:1. LI of 400 to 1600 μmol/m2 s and pH range from 7.7 to 8.7 led to the highest values of C18:3n6 (0.8{\%} DW), but C18:3n3 was suppressed by these conditions, supporting a desaturation pathway in Synechocystis. These results point to strategies to optimize LI, CO2, and pH, to enhance the fatty acid production profile for biofuel production.",
keywords = "CO, Fatty acids, Light intensity, Lipids, pH, Synechocystis",
author = "Cuellar-Bermudez, {Sara P.} and Romero-Ogawa, {Miguel A.} and Raveender Vannela and Lai, {YenJung Sean} and Bruce Rittmann and Roberto Parra-Saldivar",
year = "2015",
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T1 - Effects of light intensity and carbon dioxide on lipids and fatty acids produced by Synechocystis sp. PCC6803 during continuous flow

AU - Cuellar-Bermudez, Sara P.

AU - Romero-Ogawa, Miguel A.

AU - Vannela, Raveender

AU - Lai, YenJung Sean

AU - Rittmann, Bruce

AU - Parra-Saldivar, Roberto

PY - 2015/11/1

Y1 - 2015/11/1

N2 - We studied the effects of light intensity (LI) and CO2 supply on pH and total lipid production and fatty acids by Synechocystis sp. PCC6803 during continuous-flow operation of a photobioreactor having continuous nutrient supply. The temperature was fixed at 30 °C, and the LI pattern mimicked a day/night light cycle from 0 to 1920 μmol/m2 s. The CO2 supply varied from1 to 5% v/v of total air. The total lipid content increased proportionally to LI, reaching a high content of 14% of dry weight (DW) at the highest LI at 3% CO2. In contrast, LI had no significant influence on the total fatty acid content, which was 3.4% ± 0.5% DW, measured as fatty acid methyl esters (FAMEs). Palmitic acid (C16:0) was the main fatty acid (52% of FAMEs), but γ-linolenic acid (C18:3n6) and linoleic acid (C18:2) were significant at 20% and 14% of total FAMEs, respectively. Also, a-linolenic acid (C18:3n3), oleic acid (C18:1), and palmitoleic acid (C16:1) represented 5%, 4%, and 4% of the total FAMEs, respectively. In case of C16:0, its highest content was achieved at LI of 400 to 1500 μmol/m2 s and pH media values from 7.2 to 8.8 (3% CO2). The highest formation of C16:1 and C18:1 (desirable for biodiesel production) occurred with LI up to 600 μmol/m2 s at pH 9 (3% CO2). Stearic acid (C18:0) and linoleic acid (C18:2) contents did not vary with LI or pH, but α-linolenic acid (C18:3n3) formation occurred with patterns opposite to C18:3n6, C16:0, and C16:1. LI of 400 to 1600 μmol/m2 s and pH range from 7.7 to 8.7 led to the highest values of C18:3n6 (0.8% DW), but C18:3n3 was suppressed by these conditions, supporting a desaturation pathway in Synechocystis. These results point to strategies to optimize LI, CO2, and pH, to enhance the fatty acid production profile for biofuel production.

AB - We studied the effects of light intensity (LI) and CO2 supply on pH and total lipid production and fatty acids by Synechocystis sp. PCC6803 during continuous-flow operation of a photobioreactor having continuous nutrient supply. The temperature was fixed at 30 °C, and the LI pattern mimicked a day/night light cycle from 0 to 1920 μmol/m2 s. The CO2 supply varied from1 to 5% v/v of total air. The total lipid content increased proportionally to LI, reaching a high content of 14% of dry weight (DW) at the highest LI at 3% CO2. In contrast, LI had no significant influence on the total fatty acid content, which was 3.4% ± 0.5% DW, measured as fatty acid methyl esters (FAMEs). Palmitic acid (C16:0) was the main fatty acid (52% of FAMEs), but γ-linolenic acid (C18:3n6) and linoleic acid (C18:2) were significant at 20% and 14% of total FAMEs, respectively. Also, a-linolenic acid (C18:3n3), oleic acid (C18:1), and palmitoleic acid (C16:1) represented 5%, 4%, and 4% of the total FAMEs, respectively. In case of C16:0, its highest content was achieved at LI of 400 to 1500 μmol/m2 s and pH media values from 7.2 to 8.8 (3% CO2). The highest formation of C16:1 and C18:1 (desirable for biodiesel production) occurred with LI up to 600 μmol/m2 s at pH 9 (3% CO2). Stearic acid (C18:0) and linoleic acid (C18:2) contents did not vary with LI or pH, but α-linolenic acid (C18:3n3) formation occurred with patterns opposite to C18:3n6, C16:0, and C16:1. LI of 400 to 1600 μmol/m2 s and pH range from 7.7 to 8.7 led to the highest values of C18:3n6 (0.8% DW), but C18:3n3 was suppressed by these conditions, supporting a desaturation pathway in Synechocystis. These results point to strategies to optimize LI, CO2, and pH, to enhance the fatty acid production profile for biofuel production.

KW - CO

KW - Fatty acids

KW - Light intensity

KW - Lipids

KW - pH

KW - Synechocystis

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