Falls among industrial workers are the leading cause of work related injuries. Nowadays, many industries are opting for anti-fatigue cushioned flooring to reduce fall-related socio-economic cost. The goal of this study is to test SATECH's anti-fatigue flooring for stable gait. A pilot test with five healthy subjects (25-35 years old) has been conducted with six motion capture cameras and two force platforms embedded in a walkway to obtain kinematics and kinetics of whole body gait on two different floors. In conditions where the same floor material was used on the whole walkway, the anti-fatigue floor had smaller values of step length (p<0.01) associated with walking and lower values of required coefficient of friction (RCOF) (p<0.05). Toe clearance, heel contact velocity, whole body center of mass (COM) velocity, single foot stance, and double foot stance did not show significant differences between conditions. The results of this study suggest that subjects were to follow a more stable gait on the SATECH anti-fatigue flooring.