Effects of an experimental drug, Suberoylanilide Hydroxamic Acid (SAHA), on attachment, spreading, and stiffness of human breast cancer cells on silicon substrates

Jeannine S. Strobl, Mehdi Nikkhah, Robert Rhoades, Masoud Agah

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We are in vestigating silicon-based platforms for detection and analysis of breast cancer cells. Attachment and spreading of MDA-MB-231 hum an m etastatic breast cancer cells was compared to that of non-tumorigenic human breast epithelial cells, MCF-10A, and the impact of SAHA (Vorinostat), a histone deacetylase (HDAC) inhibitor, on cell behaviors was ascertained. Our results showed the cancer cells attached to flat silicon and PECVD nitride-coated silicon more efficiently than non-cancer cells, and preferential cancer cell attachment was enhanced by SAHA. Fluorescent immunohistochemistry (IHC) revealed that SAHA stimulated actin stress fiber formation and focal adhesion to the substrates; atomic force microscopy (AFM) showed SAHA increased the cancer cell stiffness. Collectively, SAHA-induced biomechanical changes altered the cell morphology and mode of attachment to flat silicon and to three-dimensional silicon microstructures. This is the first report of the use of AFM to characterize the biomechanical effects of a HDAC inhibitor in cancer cells.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
PublisherASME
Pages161-162
Number of pages2
ISBN (Print)9780791843925
DOIs
StatePublished - 2010
Externally publishedYes
Event1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010 - Houston, TX, United States
Duration: Feb 7 2010Feb 10 2010

Publication series

NameProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010

Other

Other1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
CountryUnited States
CityHouston, TX
Period2/7/102/10/10

ASJC Scopus subject areas

  • Medicine(all)
  • Biomedical Engineering

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    Strobl, J. S., Nikkhah, M., Rhoades, R., & Agah, M. (2010). Effects of an experimental drug, Suberoylanilide Hydroxamic Acid (SAHA), on attachment, spreading, and stiffness of human breast cancer cells on silicon substrates. In Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010 (pp. 161-162). (Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010). ASME. https://doi.org/10.1115/nemb2010-13037