Effective inhibition of influenza virus production in cultured cells by external guide sequences and ribonuclease P

Debora Plehn-Dujowich, Sidney Altman

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide sequences targeted to the mRNAs of the PB2 and NP genes are stably incorporated into mouse cells in tissue culture, infection of these cells with influenza virus is nonproductive. Endogenous RNase P cleaves the targeted influenza virus mRNAs when they are in a complex with the external guide sequences. Targeting two different mRNAs simultaneously inhibits viral particle production more efficiently than does targeting only one mRNA.

Original languageEnglish (US)
Pages (from-to)7327-7332
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number13
DOIs
StatePublished - Jun 23 1998
Externally publishedYes

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Ribonuclease P
Orthomyxoviridae
Cultured Cells
Nucleocapsid
Messenger RNA
Guide RNA
Synthetic Genes
Virus Replication
Virion
Genes
Cell Culture Techniques
Genome
Viruses
Infection

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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