TY - JOUR
T1 - Effect of ultra-violet exposure on production of mycosporine-like amino acids and lipids by Lyngbya purpurem
AU - Chandra, Rashmi
AU - Pons-Faudoa, Fernanda Paola
AU - Parra Saldívar, Roberto
AU - Rittmann, Bruce E.
N1 - Funding Information:
The authors want to thank the Director of the School of Engineering and Science, Tecnologico de Monterrey , and Biodesign Institute at Arizona State University for his encouragement and financial support. RC want to want Conacyte for proving support in form of SNI–C (CVU No. 739144 ). Author also thanks Aavesh green sustainability solutions S. De R. L. De. C. V. for their support for FTIR analysis.
Funding Information:
Spectroscopic analysis of the methanolic extract, Fig. 2a, reveals absorption peaks for UVB-absorbing compounds (absorbance between 300 and 362 nm), and this is consistent with the larger effect of UVB exposure [25]. Molecular characterization of MAAs was done by FTIR analysis and is presented in Fig. 2b. The 6 prominent bands indicate key functional groups: from left to right, 3200–3600 cm−1 for the OH group, 3100-3020 cm−1 for C–H with sp2 hybridization, 1585 cm−1 for C[dbnd]C bonds, 1342-1266 cm−1 for C–N in aromatics, 1250–1020 cm−1 for C–N in aliphatic, and 890 cm−1 for aromatics with out-of-plane ring bending. These analyses suggest that the of MAAs from Lyngbya were similar to the UV protectants porphyra-334 and shinorine [35–37]. The electron-microscope image in Section SI.2 of Supplemental Information shows that UV-B-exposed Lyngbya also had a prominent exopolymeric sheath to support the binding of the UV protectant.The authors want to thank the Director of the School of Engineering and Science, Tecnologico de Monterrey, and Biodesign Institute at Arizona State University for his encouragement and financial support. RC want to want Conacyte for proving support in form of SNI–C (CVU No. 739144). Author also thanks Aavesh green sustainability solutions S. De R. L. De. C. V. for their support for FTIR analysis.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/3
Y1 - 2020/3
N2 - Cyanobacteria can have high lipid content, are amenable to metabolic engineering, and contain valuable materials, such as ultraviolet (UV) protectants and antioxidants. Maximizing the production and harvesting of several valuable products at the same time is an important objective. UV exposure was evaluated as a strategy to induce synthesis of UV protectants, higher lipid productivity, and a high lipid-saturation index in the cyanobacterium Lyngbya purpurem UTEX LB 2716. While UVA stimulated the highest production of lipids and the largest amount of mycosporine-like amino acids (MAAs), UVB lead to greater generation of MAAs, which correlated to higher antioxidant activity. UVB also showed a shift towards saturated fatty acids, such as C16:0 and C18:0, over unsaturated fatty acids, whereas UVA showed a shift towards the unsaturated fatty acids. In summary, UVB induced the highest MAAs production, anti-oxidant activity, and lipid saturation, and its yield of crude lipids and extracted FAME was only slightly lower than with UVA.
AB - Cyanobacteria can have high lipid content, are amenable to metabolic engineering, and contain valuable materials, such as ultraviolet (UV) protectants and antioxidants. Maximizing the production and harvesting of several valuable products at the same time is an important objective. UV exposure was evaluated as a strategy to induce synthesis of UV protectants, higher lipid productivity, and a high lipid-saturation index in the cyanobacterium Lyngbya purpurem UTEX LB 2716. While UVA stimulated the highest production of lipids and the largest amount of mycosporine-like amino acids (MAAs), UVB lead to greater generation of MAAs, which correlated to higher antioxidant activity. UVB also showed a shift towards saturated fatty acids, such as C16:0 and C18:0, over unsaturated fatty acids, whereas UVA showed a shift towards the unsaturated fatty acids. In summary, UVB induced the highest MAAs production, anti-oxidant activity, and lipid saturation, and its yield of crude lipids and extracted FAME was only slightly lower than with UVA.
KW - Biodiesel
KW - Lipid saturation index
KW - Lyngbya purpurem
KW - Mass balance
KW - UV protectant
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U2 - 10.1016/j.biombioe.2020.105475
DO - 10.1016/j.biombioe.2020.105475
M3 - Article
AN - SCOPUS:85078743374
SN - 0961-9534
VL - 134
JO - Biomass and Bioenergy
JF - Biomass and Bioenergy
M1 - 105475
ER -