Effect of tethering during EPG recorded probing by adults of the greenhouse whitefly

Hong Lei, W. F. Tjallingii, J. C. Van Lenteren

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

For electrical penetration graph (EPG) recording from a piercing insect, attachment of the insect to an electrode of thin wire is needed. The effects of tethering on the probing behaviour of the greenhouse whitefly Trialeurodes vaporariorum (Westwood) were studied by comparing free (not wired) whiteflies with tethered whiteflies, using gold wire of 10 and 20 μm diameter. As an additional group, whiteflies with silver paint contact but without wiring were used to accomplish discontinuous EPG recordings. The total probing time and the total time spent with the stylers in phloem sieve elements (phloem phase) differed between these treatments. Probing time of free insects was significantly longer than that of whiteflies wired with 20 μm, but not with 10 μm wires. Time spent in phloem phase was considerably shorter for 20 than for 10 μm wired whiteflies. These differences decreased over the 8 h experimental time. In an additional 12 h experiment, a free/tethered comparison was made on the basis of honeydew excretion, using 'honeydew clock' recording. By comparing the simultaneous EPGs with honeydew recordings of wired insects, a highly positive correlation was found between total duration of phloem phases and periods of honeydew excretion. Free insects spent longer time in phloem sieve elements than wired ones and shorter intervals occurred before reaching the phloem phase, which always includes phloem feeding. However, when a 2 h adaptation period was given to the wired whiteflies, an improvement could be observed in shortening the interval to the 1st phloem phase and increasing the total time of phloem phases. Although there is still an effect of a 10 μm gold wire on probing behaviour, EPG recording seems an acceptable method for investigating probing behaviour of whiteflies, as long as control white flies in behavioural and ecophysiological studies are tethered as well. Using an adaptation procedure to reduce the wire stress is recommended.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalJournal of Applied Entomology
Volume121
Issue number4
StatePublished - Sep 1 1997
Externally publishedYes

Fingerprint

Aleyrodidae
phloem
greenhouses
wire
honeydew
insects
sieve elements
gold
excretion
Trialeurodes vaporariorum
paints
shortenings
silver
electrodes
duration

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Effect of tethering during EPG recorded probing by adults of the greenhouse whitefly. / Lei, Hong; Tjallingii, W. F.; Van Lenteren, J. C.

In: Journal of Applied Entomology, Vol. 121, No. 4, 01.09.1997, p. 211-217.

Research output: Contribution to journalArticle

Lei, Hong ; Tjallingii, W. F. ; Van Lenteren, J. C. / Effect of tethering during EPG recorded probing by adults of the greenhouse whitefly. In: Journal of Applied Entomology. 1997 ; Vol. 121, No. 4. pp. 211-217.
@article{eaf2414d999a4a8bbf9554a148d0bd9f,
title = "Effect of tethering during EPG recorded probing by adults of the greenhouse whitefly",
abstract = "For electrical penetration graph (EPG) recording from a piercing insect, attachment of the insect to an electrode of thin wire is needed. The effects of tethering on the probing behaviour of the greenhouse whitefly Trialeurodes vaporariorum (Westwood) were studied by comparing free (not wired) whiteflies with tethered whiteflies, using gold wire of 10 and 20 μm diameter. As an additional group, whiteflies with silver paint contact but without wiring were used to accomplish discontinuous EPG recordings. The total probing time and the total time spent with the stylers in phloem sieve elements (phloem phase) differed between these treatments. Probing time of free insects was significantly longer than that of whiteflies wired with 20 μm, but not with 10 μm wires. Time spent in phloem phase was considerably shorter for 20 than for 10 μm wired whiteflies. These differences decreased over the 8 h experimental time. In an additional 12 h experiment, a free/tethered comparison was made on the basis of honeydew excretion, using 'honeydew clock' recording. By comparing the simultaneous EPGs with honeydew recordings of wired insects, a highly positive correlation was found between total duration of phloem phases and periods of honeydew excretion. Free insects spent longer time in phloem sieve elements than wired ones and shorter intervals occurred before reaching the phloem phase, which always includes phloem feeding. However, when a 2 h adaptation period was given to the wired whiteflies, an improvement could be observed in shortening the interval to the 1st phloem phase and increasing the total time of phloem phases. Although there is still an effect of a 10 μm gold wire on probing behaviour, EPG recording seems an acceptable method for investigating probing behaviour of whiteflies, as long as control white flies in behavioural and ecophysiological studies are tethered as well. Using an adaptation procedure to reduce the wire stress is recommended.",
author = "Hong Lei and Tjallingii, {W. F.} and {Van Lenteren}, {J. C.}",
year = "1997",
month = "9",
day = "1",
language = "English (US)",
volume = "121",
pages = "211--217",
journal = "Journal of Applied Entomology",
issn = "0931-2048",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Effect of tethering during EPG recorded probing by adults of the greenhouse whitefly

AU - Lei, Hong

AU - Tjallingii, W. F.

AU - Van Lenteren, J. C.

PY - 1997/9/1

Y1 - 1997/9/1

N2 - For electrical penetration graph (EPG) recording from a piercing insect, attachment of the insect to an electrode of thin wire is needed. The effects of tethering on the probing behaviour of the greenhouse whitefly Trialeurodes vaporariorum (Westwood) were studied by comparing free (not wired) whiteflies with tethered whiteflies, using gold wire of 10 and 20 μm diameter. As an additional group, whiteflies with silver paint contact but without wiring were used to accomplish discontinuous EPG recordings. The total probing time and the total time spent with the stylers in phloem sieve elements (phloem phase) differed between these treatments. Probing time of free insects was significantly longer than that of whiteflies wired with 20 μm, but not with 10 μm wires. Time spent in phloem phase was considerably shorter for 20 than for 10 μm wired whiteflies. These differences decreased over the 8 h experimental time. In an additional 12 h experiment, a free/tethered comparison was made on the basis of honeydew excretion, using 'honeydew clock' recording. By comparing the simultaneous EPGs with honeydew recordings of wired insects, a highly positive correlation was found between total duration of phloem phases and periods of honeydew excretion. Free insects spent longer time in phloem sieve elements than wired ones and shorter intervals occurred before reaching the phloem phase, which always includes phloem feeding. However, when a 2 h adaptation period was given to the wired whiteflies, an improvement could be observed in shortening the interval to the 1st phloem phase and increasing the total time of phloem phases. Although there is still an effect of a 10 μm gold wire on probing behaviour, EPG recording seems an acceptable method for investigating probing behaviour of whiteflies, as long as control white flies in behavioural and ecophysiological studies are tethered as well. Using an adaptation procedure to reduce the wire stress is recommended.

AB - For electrical penetration graph (EPG) recording from a piercing insect, attachment of the insect to an electrode of thin wire is needed. The effects of tethering on the probing behaviour of the greenhouse whitefly Trialeurodes vaporariorum (Westwood) were studied by comparing free (not wired) whiteflies with tethered whiteflies, using gold wire of 10 and 20 μm diameter. As an additional group, whiteflies with silver paint contact but without wiring were used to accomplish discontinuous EPG recordings. The total probing time and the total time spent with the stylers in phloem sieve elements (phloem phase) differed between these treatments. Probing time of free insects was significantly longer than that of whiteflies wired with 20 μm, but not with 10 μm wires. Time spent in phloem phase was considerably shorter for 20 than for 10 μm wired whiteflies. These differences decreased over the 8 h experimental time. In an additional 12 h experiment, a free/tethered comparison was made on the basis of honeydew excretion, using 'honeydew clock' recording. By comparing the simultaneous EPGs with honeydew recordings of wired insects, a highly positive correlation was found between total duration of phloem phases and periods of honeydew excretion. Free insects spent longer time in phloem sieve elements than wired ones and shorter intervals occurred before reaching the phloem phase, which always includes phloem feeding. However, when a 2 h adaptation period was given to the wired whiteflies, an improvement could be observed in shortening the interval to the 1st phloem phase and increasing the total time of phloem phases. Although there is still an effect of a 10 μm gold wire on probing behaviour, EPG recording seems an acceptable method for investigating probing behaviour of whiteflies, as long as control white flies in behavioural and ecophysiological studies are tethered as well. Using an adaptation procedure to reduce the wire stress is recommended.

UR - http://www.scopus.com/inward/record.url?scp=0030827307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030827307&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030827307

VL - 121

SP - 211

EP - 217

JO - Journal of Applied Entomology

JF - Journal of Applied Entomology

SN - 0931-2048

IS - 4

ER -