Effect of selected herbicides on growth and hydrocarbon content of Botryococcus braunii (Race B)

Liping Deng, Scott A. Senseman, Terry J. Gentry, David A. Zuberer, Taylor Weiss, Timothy P. Devarenne, Edinalvo R. Camargo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The algae Botryococcus braunii, one of the potential renewable resources for production of liquid hydrocarbons, was used for testing the effect of selected herbicides on algal growth and hydrocarbon content. Twenty-two herbicides representing 14 modes of action were assayed. Accelerated solvent extraction (ASE) was used to extract hydrocarbon from algae and the results showed that ASE was an efficient method for algal hydrocarbon extraction at 50. °C extraction temperature, 10.3. MPa of pressure and 1 static cycle using n-hexane as extraction solvent. The photosystem II inhibitor diuron was the most toxic herbicide for algal growth, but hydrocarbon content increased from 34.9 to 42.4% of dry biomass in the presence of a 0.1. mg/L concentration of diuron. The photosystem I inhibitor diquat was inhibitory to growth of B. braunii, but hydrocarbon content increased to 43.3% of dry biomass when treated with 5. mg/L diquat. S-metalochlor, a mitosis inhibitor, reduced both algal growth and hydrocarbon content. Hydrocarbon content decreased to 16.4, 14.0 and 5.5% of dry biomass, respectively when treated with 0.1, 1, and 5. mg/L S-metalochlor. The carotenoid biosynthesis inhibitor fluridone did not affect algal growth at 0.1. mg/L, but decreased hydrocarbon content from 34.9 to 13.2%. The fatty acid and lipid biosynthesis inhibitor thiobencarb had no effect on algal growth or hydrocarbon content at 0.1. mg/L, but it inhibited algal growth and decreased hydrocarbon content from 34.9 to 7.8% at 1. mg/L. The oxidative phosphorylation uncoupler dinoterb at 0.1. mg/L reduced algal biomass by 52.8% and decreased hydrocarbon content from 34.9 to 30.0%. The use of B. braunii as a source of fuel will undoubtedly require the use of an outdoor open pond system. These results will be useful for improving algal oil production while developing management systems to control invasive algal species in outdoor open pond systems ultimately keeping the oil producing algae pure.

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalIndustrial Crops and Products
Volume39
Issue number1
DOIs
StatePublished - Sep 1 2012
Externally publishedYes

Fingerprint

hydrocarbons
herbicides
algae
diquat
diuron
biomass
dinoterb
Botryococcus braunii
thiobencarb
biosynthesis
fluridone
renewable resources
oxidative phosphorylation
photosystem I
hexane
mitosis
photosystem II
management systems
mechanism of action
carotenoids

Keywords

  • Accelerated solvent extraction
  • Botryococcus braunii
  • Herbicides
  • Hydrocarbon

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Deng, L., Senseman, S. A., Gentry, T. J., Zuberer, D. A., Weiss, T., Devarenne, T. P., & Camargo, E. R. (2012). Effect of selected herbicides on growth and hydrocarbon content of Botryococcus braunii (Race B). Industrial Crops and Products, 39(1), 154-161. https://doi.org/10.1016/j.indcrop.2012.02.031

Effect of selected herbicides on growth and hydrocarbon content of Botryococcus braunii (Race B). / Deng, Liping; Senseman, Scott A.; Gentry, Terry J.; Zuberer, David A.; Weiss, Taylor; Devarenne, Timothy P.; Camargo, Edinalvo R.

In: Industrial Crops and Products, Vol. 39, No. 1, 01.09.2012, p. 154-161.

Research output: Contribution to journalArticle

Deng, Liping ; Senseman, Scott A. ; Gentry, Terry J. ; Zuberer, David A. ; Weiss, Taylor ; Devarenne, Timothy P. ; Camargo, Edinalvo R. / Effect of selected herbicides on growth and hydrocarbon content of Botryococcus braunii (Race B). In: Industrial Crops and Products. 2012 ; Vol. 39, No. 1. pp. 154-161.
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