Effect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae)

Yantao Li, Milton Sommerfeld, Feng Chen, Qiang Hu

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The green alga Haematococcus pluvialis produces large amounts of the pink carotenoid astaxanthin under high photon flux density (PFD) and other oxidative stress conditions. However, the regulation and physiological role of carotenogenesis leading to astaxanthin formation is not well understood. Comparative transcriptional expression of five carotenoid genes along with growth and pigment composition as a function of PFD was studied using a wild-type and an astaxanthin-overproduction mutant of H. pluvialis NIES144. The results indicate that astaxanthin biosynthesis was mainly under transcriptional control of the gene encoding carotenoid hydroxylase, and to a lesser extent, the genes encoding isopentenyl isomerase and phytoene desaturase, and to the least extent, the genes encoding phytoene synthase and carotenoid oxygenase. The expression of a plastid terminal oxidase (PTOX) gene ptox2 underwent transient up-regulation under elevated PFDs, suggesting that PTOX may be functionally coupled with phytoene desaturase through the plastoquinone pool and may play a role in reducing redox-potential-dependent and oxygen-concentration-dependent formation of reactive oxygen species in the chloroplast. Over-expression of both the carotenogenic and PTOX genes confers to the astaxanthin-overproduction mutant more effective photoprotective capability than that of the wild type under photooxidative stress.

Original languageEnglish (US)
Pages (from-to)253-263
Number of pages11
JournalJournal of Applied Phycology
Volume22
Issue number3
DOIs
StatePublished - Jun 2010
Externally publishedYes

Fingerprint

Haematococcus pluvialis
photon flux density
Chlorophyceae
astaxanthin
cell viability
viability
carotenoid
gene
plastid
carotenoids
plastids
genes
phytoene synthase
mutants
isomerases
redox potential
oxygenases
green alga
chloroplast
Chlorophyta

Keywords

  • Astaxanthin
  • Haematococcus pluvialis
  • High light
  • mRNA expression
  • Oxidative stress

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Effect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae). / Li, Yantao; Sommerfeld, Milton; Chen, Feng; Hu, Qiang.

In: Journal of Applied Phycology, Vol. 22, No. 3, 06.2010, p. 253-263.

Research output: Contribution to journalArticle

Li, Yantao ; Sommerfeld, Milton ; Chen, Feng ; Hu, Qiang. / Effect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae). In: Journal of Applied Phycology. 2010 ; Vol. 22, No. 3. pp. 253-263.
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