Effect of phenobarbital administration to rats on the level of the in vitro synthesis of cytochrome P-450 directed by total rat liver RNA

Raymond N. Dubois, Michael R. Waterman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Total liver RNA has been isolated from male rats at different time points subsequent to a single injection of phenobarbital, and the level of cytochrome P-450 synthesis directed by these RNA preparations in a cell-free translation system has been determined. It is observed that the maximum in vitro synthesis of cytochrome P-450 occurs at 16 hours (3-fold above uninduced level) which is approximately 30 hours prior to the maximum induction of spectrophotometrically detectable cytochrome P-450 measured in liver homogenates. Thus, while cytochrome P-450 mRNA is involved in the induction process, its synthesis does not appear to be rate limiting. In addition, phenobarbital induced cytochrome P-450 is not synthesized in vitro in a form larger than that isolated from endoplasmic reticulum, but rather is also found to have a molecular weight of 50,000.

Original languageEnglish (US)
Pages (from-to)150-157
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume90
Issue number1
DOIs
StatePublished - Sep 12 1979
Externally publishedYes

Fingerprint

Phenobarbital
Liver
Cytochrome P-450 Enzyme System
Rats
RNA
Cell-Free System
Endoplasmic Reticulum
Molecular Weight
Molecular weight
In Vitro Techniques
Messenger RNA
Injections

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of phenobarbital administration to rats on the level of the in vitro synthesis of cytochrome P-450 directed by total rat liver RNA. / Dubois, Raymond N.; Waterman, Michael R.

In: Biochemical and Biophysical Research Communications, Vol. 90, No. 1, 12.09.1979, p. 150-157.

Research output: Contribution to journalArticle

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