Effect of Hepatitis C Infection on HIV-Induced Apoptosis

Tomasz Laskus, Karen Kibler, Marcin Chmielewski, Jeffrey Wilkinson, Debra Adair, Andrzej Horban, Grzegorz Stańczak, Marek Radkowski

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background:Hepatitis C virus (HCV) coinfection was reported to negatively affect HIV disease and HIV infection has a deleterious effect on HCV-related liver disease. However, despite common occurrence of HCV/HIV coinfection little is known about the mechanisms of interactions between the two viruses.Methods:We studied CD4+ and CD8+ T cell and CD19+ B cell apoptosis in 104 HIV-positive patients (56 were also HCV-positive) and in 22 HCV/HIV-coinfected patients treated for chronic hepatitis C with pegylated interferon and ribavirin. We also analyzed HCV/HIV coinfection in a Daudi B-cell line expressing CD4 and susceptible to both HCV and HIV infection. Apoptosis was measured by AnnexinV staining.Results:HCV/HIV coinfected patients had lower CD4+ and CD8+ T cell apoptosis and higher CD19+ B cell apoptosis than those with HIV monoinfection. Furthermore, anti-HCV treatment of HCV/HIV coinfected patients was followed by an increase of CD4+ and CD8+ T cell apoptosis and a decrease of CD19+ B cell apoptosis. In the Daudi CD4+ cell line, presence of HCV infection facilitated HIV replication, however, decreased the rate of HIV-related cell death.Conclusion:In HCV/HIV coinfected patients T-cells were found to be destroyed at a slower rate than in HIV monoinfected patients. These results suggest that HCV is a molecular-level determinant in HIV disease.

Original languageEnglish (US)
Article numbere75921
JournalPLoS One
Volume8
Issue number10
DOIs
StatePublished - Oct 1 2013

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hepatitis C
Hepatitis C virus
Hepatitis C
Viruses
Hepacivirus
HIV Infections
apoptosis
HIV
Apoptosis
infection
T-cells
B-lymphocytes
Cells
B-Lymphocytes
T-lymphocytes
Coinfection
mixed infection
T-Lymphocytes
HIV infections
Virus Diseases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Laskus, T., Kibler, K., Chmielewski, M., Wilkinson, J., Adair, D., Horban, A., ... Radkowski, M. (2013). Effect of Hepatitis C Infection on HIV-Induced Apoptosis. PLoS One, 8(10), [e75921]. https://doi.org/10.1371/journal.pone.0075921

Effect of Hepatitis C Infection on HIV-Induced Apoptosis. / Laskus, Tomasz; Kibler, Karen; Chmielewski, Marcin; Wilkinson, Jeffrey; Adair, Debra; Horban, Andrzej; Stańczak, Grzegorz; Radkowski, Marek.

In: PLoS One, Vol. 8, No. 10, e75921, 01.10.2013.

Research output: Contribution to journalArticle

Laskus, T, Kibler, K, Chmielewski, M, Wilkinson, J, Adair, D, Horban, A, Stańczak, G & Radkowski, M 2013, 'Effect of Hepatitis C Infection on HIV-Induced Apoptosis', PLoS One, vol. 8, no. 10, e75921. https://doi.org/10.1371/journal.pone.0075921
Laskus T, Kibler K, Chmielewski M, Wilkinson J, Adair D, Horban A et al. Effect of Hepatitis C Infection on HIV-Induced Apoptosis. PLoS One. 2013 Oct 1;8(10). e75921. https://doi.org/10.1371/journal.pone.0075921
Laskus, Tomasz ; Kibler, Karen ; Chmielewski, Marcin ; Wilkinson, Jeffrey ; Adair, Debra ; Horban, Andrzej ; Stańczak, Grzegorz ; Radkowski, Marek. / Effect of Hepatitis C Infection on HIV-Induced Apoptosis. In: PLoS One. 2013 ; Vol. 8, No. 10.
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