Effect of extremely high-CO2 stress on energy distribution between photosystem I and photosystem II in a 'high-CO2' tolerant green alga, Chlorococcum littorale and the intolerant green alga Stichococcus bacillaris

Ikuko Iwasaki, Qiang Hu, Norihide Kurano, Shigetoh Miyachi

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

A green alga, Chlorococcum littorale, has a tolerance to extremely high-CO2 conditions. In order to elucidate the mechanism underlying the resistance to such high CO2 levels, we compared the changes in excitation energy distribution between photosystem I (PS I) and photosystem II (PS II) by 77 K fluorescence in cells of the high CO2-resistant C. littorale and the non-resistant Stichococcus bacillaris. Immediately after the cells are transferred from air to 40% CO2, the F714/F687 ratio derived from 77 K fluorescence increases in C. littorale cells, suggesting an increase of transition from state 1 to state 2. During this period, more than 80% of plastoquinone A is in the reduced form and the activity of PS I increases. Eventually the F714/F687 ratio, the concentration of reduced plastoquinone A and PS I activity decrease. However, no significant increase of the F714/F687 ratio is observed after the transfer of S.bacillaris cells from air to 40% CO2. The level of reduced plastoquinone A in S. bacillaris gradually increases and the activity of PS I does not show a large change. During the transient period, the level of the D1 protein is approximately constant in C. littorale cells, but is lowered in S. bacillaris. These results suggest that, under extremely high-CO2 conditions, PS II is protected from photoinhibition by control of the state transition in C. littorale cells, whereas such a protection mechanism does not function in the alga S. bacillaris, non-resistant to CO2.

Original languageEnglish (US)
Pages (from-to)184-190
Number of pages7
JournalJournal of Photochemistry and Photobiology B: Biology
Volume44
Issue number3
DOIs
StatePublished - Jul 31 1998

Keywords

  • CO concentration
  • Chlorococcum littorale
  • Chlorophyll fluorescence
  • Photoinhibition
  • Photosystem I
  • Photosystem II
  • Plastoquinone A
  • Quantum yield
  • State transition

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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