Effect of environmental variables on eukaryotic microbial community structure of land-fast Arctic sea ice

Brian Eddie, Andrew Juhl, Christopher Krembs, Charles Baysinger, Susanne Neuer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenting the composition of the community in relation to vertical depth within the cores, as well as light availability (mainly as variable snow cover) and nutrient concentrations. We applied a combination of epifluorescence microscopy, denaturing gradient gel electrophoresis and clone libraries of a section of the 18S rRNA gene in order to compare the community structure of the major eukaryotic microbial phylotypes in the ice. We find that the community composition of the sea ice is more affected by the depth horizon in the ice than by light availability, although there are significant differences in the abundance of some groups between light regimes. Epifluorescence microscopy shows a shift from predominantly heterotrophic life styles in the upper ice to autotrophy prevailing in the bottom ice. This is supported by the statistical analysis of the similarity between the samples based on the denaturing gradient gel electrophoresis banding patterns, which shows a clear difference between upper and lower ice sections with respect to phylotypes and their proportional abundance. Clone libraries constructed using diatom-specific primers confirm the high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.

Original languageEnglish (US)
Pages (from-to)797-809
Number of pages13
JournalEnvironmental Microbiology
Volume12
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Ice Cover
Ice
Diatoms
microbial communities
sea ice
Arctic region
microbial community
community structure
ice
environmental factors
diatom
Bacillariophyceae
Food
Denaturing Gradient Gel Electrophoresis
Carbon
light availability
Light
nutrient
microscopy
clone

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Effect of environmental variables on eukaryotic microbial community structure of land-fast Arctic sea ice. / Eddie, Brian; Juhl, Andrew; Krembs, Christopher; Baysinger, Charles; Neuer, Susanne.

In: Environmental Microbiology, Vol. 12, No. 3, 03.2010, p. 797-809.

Research output: Contribution to journalArticle

Eddie, Brian ; Juhl, Andrew ; Krembs, Christopher ; Baysinger, Charles ; Neuer, Susanne. / Effect of environmental variables on eukaryotic microbial community structure of land-fast Arctic sea ice. In: Environmental Microbiology. 2010 ; Vol. 12, No. 3. pp. 797-809.
@article{77240fb2e4844b488a70bc222385adc2,
title = "Effect of environmental variables on eukaryotic microbial community structure of land-fast Arctic sea ice",
abstract = "Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenting the composition of the community in relation to vertical depth within the cores, as well as light availability (mainly as variable snow cover) and nutrient concentrations. We applied a combination of epifluorescence microscopy, denaturing gradient gel electrophoresis and clone libraries of a section of the 18S rRNA gene in order to compare the community structure of the major eukaryotic microbial phylotypes in the ice. We find that the community composition of the sea ice is more affected by the depth horizon in the ice than by light availability, although there are significant differences in the abundance of some groups between light regimes. Epifluorescence microscopy shows a shift from predominantly heterotrophic life styles in the upper ice to autotrophy prevailing in the bottom ice. This is supported by the statistical analysis of the similarity between the samples based on the denaturing gradient gel electrophoresis banding patterns, which shows a clear difference between upper and lower ice sections with respect to phylotypes and their proportional abundance. Clone libraries constructed using diatom-specific primers confirm the high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.",
author = "Brian Eddie and Andrew Juhl and Christopher Krembs and Charles Baysinger and Susanne Neuer",
year = "2010",
month = "3",
doi = "10.1111/j.1462-2920.2009.02126.x",
language = "English (US)",
volume = "12",
pages = "797--809",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Effect of environmental variables on eukaryotic microbial community structure of land-fast Arctic sea ice

AU - Eddie, Brian

AU - Juhl, Andrew

AU - Krembs, Christopher

AU - Baysinger, Charles

AU - Neuer, Susanne

PY - 2010/3

Y1 - 2010/3

N2 - Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenting the composition of the community in relation to vertical depth within the cores, as well as light availability (mainly as variable snow cover) and nutrient concentrations. We applied a combination of epifluorescence microscopy, denaturing gradient gel electrophoresis and clone libraries of a section of the 18S rRNA gene in order to compare the community structure of the major eukaryotic microbial phylotypes in the ice. We find that the community composition of the sea ice is more affected by the depth horizon in the ice than by light availability, although there are significant differences in the abundance of some groups between light regimes. Epifluorescence microscopy shows a shift from predominantly heterotrophic life styles in the upper ice to autotrophy prevailing in the bottom ice. This is supported by the statistical analysis of the similarity between the samples based on the denaturing gradient gel electrophoresis banding patterns, which shows a clear difference between upper and lower ice sections with respect to phylotypes and their proportional abundance. Clone libraries constructed using diatom-specific primers confirm the high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.

AB - Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenting the composition of the community in relation to vertical depth within the cores, as well as light availability (mainly as variable snow cover) and nutrient concentrations. We applied a combination of epifluorescence microscopy, denaturing gradient gel electrophoresis and clone libraries of a section of the 18S rRNA gene in order to compare the community structure of the major eukaryotic microbial phylotypes in the ice. We find that the community composition of the sea ice is more affected by the depth horizon in the ice than by light availability, although there are significant differences in the abundance of some groups between light regimes. Epifluorescence microscopy shows a shift from predominantly heterotrophic life styles in the upper ice to autotrophy prevailing in the bottom ice. This is supported by the statistical analysis of the similarity between the samples based on the denaturing gradient gel electrophoresis banding patterns, which shows a clear difference between upper and lower ice sections with respect to phylotypes and their proportional abundance. Clone libraries constructed using diatom-specific primers confirm the high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice.

UR - http://www.scopus.com/inward/record.url?scp=77953921576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953921576&partnerID=8YFLogxK

U2 - 10.1111/j.1462-2920.2009.02126.x

DO - 10.1111/j.1462-2920.2009.02126.x

M3 - Article

C2 - 20050870

AN - SCOPUS:77953921576

VL - 12

SP - 797

EP - 809

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 3

ER -