Effect of ascorbic acid nutriture on protein-bound hydroxyproline in guinea pig plasma

Carol Johnston, G. D. Cartee, B. E. Haskell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This paper provides indirect evidence that ascorbate nutriture affects plasma concentrations of complement component C1q in the guinea pig. C1q is a protein with a hydroxyproline-rich region similar in structure to collagen. It is essential for complement-mediated lysis of pathogens and may also facilitate phagocytic activity of macrophages and neutrophils. Since C1q is the only hydroxyproline-containing protein in the euglobulin fraction of plasma, it can be quantified indirectly by precipitating this fraction, hydrolyzing it and estimating hydroxyproline colorimetrically. We investigated the effect of ascorbate nutriture on protein-bound hydroxyproline (PBH) in the euglobulin fraction of plasma of young male guinea pigs. The animals had been depleted of ascorbate for 3 wk to produce scurvy and then repleted (6 wk) as follows: 0.5, 2.0 and 10.0 mg ascorbate/100 g body weight per d or 10 g ascorbate per liter of drinking water. PBH values were significantly correlated (P < 0.001) with dietary ascorbate (+0.74) and with liver ascorbate (+0.75). Plasma PBH was significantly higher (P < 0.01, Scheffe's test) in guinea pigs fed ample ascorbate (10.0 mg/100 g body weight per day) or tissue-saturating levels (10 g/L of drinking water) than in those fed adequate (2.0 mg/100 g body weight) or suboptimal (0.5 mg/100 g body weight) levels. These data are consistent with the known biochemical role of ascorbic acid in hydroxyproline biosynthesis and suggest a possible link between ascorbate and the immune response via C1q.

Original languageEnglish (US)
Pages (from-to)1089-1093
Number of pages5
JournalJournal of Nutrition
Volume115
Issue number8
StatePublished - 1985
Externally publishedYes

Fingerprint

hydroxyproline
Hydroxyproline
guinea pigs
Ascorbic Acid
Guinea Pigs
ascorbic acid
Body Weight
Proteins
proteins
Serum Globulins
body weight
Drinking Water
drinking water
complement
Complement C1q
scurvy
Scurvy
phagocytosis
blood proteins
collagen

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Effect of ascorbic acid nutriture on protein-bound hydroxyproline in guinea pig plasma. / Johnston, Carol; Cartee, G. D.; Haskell, B. E.

In: Journal of Nutrition, Vol. 115, No. 8, 1985, p. 1089-1093.

Research output: Contribution to journalArticle

Johnston, Carol ; Cartee, G. D. ; Haskell, B. E. / Effect of ascorbic acid nutriture on protein-bound hydroxyproline in guinea pig plasma. In: Journal of Nutrition. 1985 ; Vol. 115, No. 8. pp. 1089-1093.
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