Effect of a polymorphism in the ND1 mitochondrial gene on human skeletal muscle mitochondrial function

Matthew R. Jackman, E. Ravussin, M. J. Rowe, R. Pratley, M. R. Milner, W. T. Willis

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Objective:A non-silent polymorphism in the mitochondrial coding region of the ND1 gene, a subunit of reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase is associated with resting metabolic rate (RMR) in 245 non-diabetic Pima Indians. The purpose of this investigation was to determine the effect of the ND1 gene polymorphism on mitochondrial function in 14 male Pima Indians.Methods and Procedures:Seven subjects with an A at site 3547 of the ND1 gene (Ile at amino acid 81), and seven with a G at this site (Val) were studied. Mitochondria were isolated from 0.8 to 1.5 g of skeletal muscle obtained by needle biopsy of the lateral quadriceps muscle. In intact mitochondria, maximal (state-3) and resting (state-4) respiration rates were measured polarographically at 37 °C with a variety of single substrates or substrate combinations. Disrupted mitochondria were analyzed for maximal capacities through the entire electron transport chain (ETC) (NADH oxidase (NADHOX)), as well as through a segment of Complex I that is independent of the ND1 component (NADH-ferricyanide (NADH-FeCN) reductase).Results:Mitochondria were well coupled and exhibited higher respiratory control ratios (RCRs) than rodent muscle. There were no differences between the two groups for any of the measured parameters.Discussion:These results indicate that the cause of the observed association between RMR and the ND1 polymorphism is not related to in vitro mitochondrial function.

Original languageEnglish (US)
Pages (from-to)363-368
Number of pages6
JournalObesity
Volume16
Issue number2
DOIs
StatePublished - Feb 2008

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

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    Jackman, M. R., Ravussin, E., Rowe, M. J., Pratley, R., Milner, M. R., & Willis, W. T. (2008). Effect of a polymorphism in the ND1 mitochondrial gene on human skeletal muscle mitochondrial function. Obesity, 16(2), 363-368. https://doi.org/10.1038/oby.2007.40