Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT)

Potatoes expressing a synthetic LT-B gene

Hugh Mason, Haq Tariq A, John D. Clements, Charles J. Arntzen

Research output: Contribution to journalArticle

283 Citations (Scopus)

Abstract

The authors have designed and constructed a plant-optimized synthetic gene encoding the Escherichia coli heat-labile enterotoxin B subunit (LT-B), for use in transgenic plants as an edible vaccine against enterotoxigenic E. coli. Expression of the synthetic LT-B gene in potato plants under the control of a constitutive promoter yielded increased accumulation of LT-B in leaves and tubers, as compared to the bacterial LT-B gene. The plant-derived LT-B assembled into native pentameric structures as evidenced by its ability to bind ganglioside. The authors demonstrated immunogenicity by feeding mice the raw tubers and comparing the anti-LT-B serum IgG and faecal IgA to that produced in mice gavaged with bacterial LT-B. Mice were fed three weekly doses of 5 g tuber tissue containing either 20 or 50 μg LT-B, or gavaged weekly with 5 μg of LT-B from recombinant E. coli. One week after the third dose, mice immunized with potato LT-B had higher levels of serum and mucosal anti-LT-B than those garaged with bacterial LT-B. Mice were challenged by oral administration of 25 μg LT, and protection assessed by comparing the gut/carcass mass ratios. Although none of the mice were completely protected, the higher dose potato vaccine compared favourably with the bacterial vaccine. These findings show that an edible vaccine against E. coli LT-B is feasible.

Original languageEnglish (US)
Pages (from-to)1336-1343
Number of pages8
JournalVaccine
Volume16
Issue number13
DOIs
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Edible Vaccines
edible vaccines
Enterotoxins
enterotoxins
Solanum tuberosum
Hot Temperature
potatoes
Escherichia coli
heat
mice
Genes
tubers
genes
dosage
Escherichia coli B
Bacterial Vaccines
Synthetic Genes
synthetic genes
Enterotoxigenic Escherichia coli
gangliosides

Keywords

  • Diarrhoeal-disease
  • E. coli
  • Enterotoxin-LT-B
  • Mouse
  • Plant-vaccine
  • Potato

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT) : Potatoes expressing a synthetic LT-B gene. / Mason, Hugh; Tariq A, Haq; Clements, John D.; Arntzen, Charles J.

In: Vaccine, Vol. 16, No. 13, 08.1998, p. 1336-1343.

Research output: Contribution to journalArticle

Mason, Hugh ; Tariq A, Haq ; Clements, John D. ; Arntzen, Charles J. / Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT) : Potatoes expressing a synthetic LT-B gene. In: Vaccine. 1998 ; Vol. 16, No. 13. pp. 1336-1343.
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