Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer's β-amyloid

Mathumai Kanapathipillai, Georg Lentzen, Michael Sierks, Chan Beum Park

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

β-Amyloid peptide (Aβ) is the major constituent of senile plaques, the key pathological feature of Alzheimer's disease. Aβ is physiologically produced as a soluble form, but aggregation of Aβ monomers into oligomers/fibrils causes neurotoxic change of the peptide. In nature, many microorganisms accumulate small molecule chaperones (SMCs) under stressful conditions to prevent the misfolding/denaturation of proteins and to maintain their stability. Hence, it is conceivable that SMCs such as ectoine and hydroxyectoine could be potential inhibitors against the aggregate formation of Alzheimer's Aβ, which has not been studied to date. The current work shows the effectiveness of ectoine and hydroxyectoine on the inhibition of Aβ42 aggregation and toxicity to human neuroblastoma cells. The characterization tools used for this study include thioflavin-T induced fluorescence, atomic force microscopy and cell viability assay. Considering that ectoine and hydroxyectoine are not toxic to cellular environment even at concentrations as high as 100 mM, the results may suggest a basis for the development of ectoines as potential inhibitors associated with neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)4775-4780
Number of pages6
JournalFEBS Letters
Volume579
Issue number21
DOIs
StatePublished - Aug 29 2005

Fingerprint

Amyloid
Agglomeration
Neurodegenerative diseases
Protein Denaturation
Denaturation
Molecules
Atomic Force Microscopy
Poisons
Amyloid Plaques
Neuroblastoma
Oligomers
Neurodegenerative Diseases
Microorganisms
Toxicity
Assays
Atomic force microscopy
Cell Survival
Alzheimer Disease
Monomers
Fluorescence

Keywords

  • β-Amyloid peptide
  • Alzheimer's disease
  • Ectoine
  • Hydroxyectoine
  • Small molecule chaperones

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer's β-amyloid. / Kanapathipillai, Mathumai; Lentzen, Georg; Sierks, Michael; Park, Chan Beum.

In: FEBS Letters, Vol. 579, No. 21, 29.08.2005, p. 4775-4780.

Research output: Contribution to journalArticle

Kanapathipillai, Mathumai ; Lentzen, Georg ; Sierks, Michael ; Park, Chan Beum. / Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer's β-amyloid. In: FEBS Letters. 2005 ; Vol. 579, No. 21. pp. 4775-4780.
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