Eccrine Sweat as a Biofluid for Profiling Immune Biomarkers

Benjamin A. Katchman, Meilin Zhu, Jennifer Blain Christen, Karen Anderson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Sweat is a relatively unexplored biofluid for diagnosis and monitoring of disease states. In this study, the proteomic profiling of immune-related biomarkers from healthy individuals are presented. Experimental Design: Eccrine sweat samples are collected from 50 healthy individuals. LC-MS/MS is performed on two pools of sweat samples from five male and female participants. Individual sweat samples are analyzed by antibody isotyping microarrays (n = 49), human cytokine arrays (n = 30), and quantitative ELISAs for interleukin-1α (n = 16), epidermal growth factor (n = 6), and angiogenin (n = 7). Results: In sweat, 220 unique proteins are identified by shotgun analysis. Detectable antibody isotypes include IgA (100% positive; median 1230 ± 28 700 pg mL−1), IgD (18%; 22.0 ± 119 pg mL−1), IgG1 (96%; 1640 ± 6750 pg mL−1), IgG2 (37%; 292 ± 6810 pg mL−1), IgG3 (71%; 74.0 ± 119 pg mL−1), IgG4 (69%; 43.0 ± 42.0 pg mL−1), and IgM (41%; 69.0 ± 1630 pg mL−1). Of 42 cytokines, three are readily detected in all sweat samples (p < 0.01). The median concentration for interleukin-1α is 352 ± 521 pg mL−1, epidermal growth factor is 86.5 ± 147 pg mL−1, and angiogenin is 38.3 ± 96.3 pg mL−1. Multiple other cytokines are detected at lower levels. Conclusions and Clinical Relevance: Sweat can be used for profiling antibodies and innate immune biomarkers.

Original languageEnglish (US)
Article number1800010
JournalProteomics - Clinical Applications
Volume12
Issue number6
DOIs
StatePublished - Nov 1 2018

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Sweat
Biomarkers
Immunoglobulin G
Cytokines
Interleukin-1
Epidermal Growth Factor
Antibodies
Immunoglobulin D
Microarrays
Design of experiments
Immunoglobulin A
Immunoglobulin M
Firearms
Monitoring
Proteomics
Research Design
Enzyme-Linked Immunosorbent Assay
Proteins
angiogenin

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Eccrine Sweat as a Biofluid for Profiling Immune Biomarkers. / Katchman, Benjamin A.; Zhu, Meilin; Blain Christen, Jennifer; Anderson, Karen.

In: Proteomics - Clinical Applications, Vol. 12, No. 6, 1800010, 01.11.2018.

Research output: Contribution to journalArticle

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abstract = "Purpose: Sweat is a relatively unexplored biofluid for diagnosis and monitoring of disease states. In this study, the proteomic profiling of immune-related biomarkers from healthy individuals are presented. Experimental Design: Eccrine sweat samples are collected from 50 healthy individuals. LC-MS/MS is performed on two pools of sweat samples from five male and female participants. Individual sweat samples are analyzed by antibody isotyping microarrays (n = 49), human cytokine arrays (n = 30), and quantitative ELISAs for interleukin-1α (n = 16), epidermal growth factor (n = 6), and angiogenin (n = 7). Results: In sweat, 220 unique proteins are identified by shotgun analysis. Detectable antibody isotypes include IgA (100{\%} positive; median 1230 ± 28 700 pg mL−1), IgD (18{\%}; 22.0 ± 119 pg mL−1), IgG1 (96{\%}; 1640 ± 6750 pg mL−1), IgG2 (37{\%}; 292 ± 6810 pg mL−1), IgG3 (71{\%}; 74.0 ± 119 pg mL−1), IgG4 (69{\%}; 43.0 ± 42.0 pg mL−1), and IgM (41{\%}; 69.0 ± 1630 pg mL−1). Of 42 cytokines, three are readily detected in all sweat samples (p < 0.01). The median concentration for interleukin-1α is 352 ± 521 pg mL−1, epidermal growth factor is 86.5 ± 147 pg mL−1, and angiogenin is 38.3 ± 96.3 pg mL−1. Multiple other cytokines are detected at lower levels. Conclusions and Clinical Relevance: Sweat can be used for profiling antibodies and innate immune biomarkers.",
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