Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust

Lara Rajeev, Ulisses Nunes Da Rocha, Niels Klitgord, Eric G. Luning, Julian Fortney, Seth D. Axen, Patrick M. Shih, Nicholas J. Bouskill, Benjamin P. Bowen, Cheryl A. Kerfeld, Ferran Garcia-Pichel, Eoin L. Brodie, Trent R. Northen, Aindrila Mukhopadhyay

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Biological soil crusts (BSCs) cover extensive portions of the earth's deserts. In order to survive desiccation cycles and utilize short periods of activity during infrequent precipitation, crust microorganisms must rely on the unique capabilities of vegetative cells to enter a dormant state and be poised for rapid resuscitation upon wetting. To elucidate the key events involved in the exit from dormancy, we performed a wetting experiment of a BSC and followed the response of the dominant cyanobacterium, Microcoleus vaginatus, in situ using a whole-genome transcriptional time course that included two diel cycles. Immediate, but transient, induction of DNA repair and regulatory genes signaled the hydration event. Recovery of photosynthesis occurred within 1 h, accompanied by upregulation of anabolic pathways. Onset of desiccation was characterized by the induction of genes for oxidative and photo-oxidative stress responses, osmotic stress response and the synthesis of C and N storage polymers. Early expression of genes for the production of exopolysaccharides, additional storage molecules and genes for membrane unsaturation occurred before drying and hints at preparedness for desiccation. We also observed signatures of preparation for future precipitation, notably the expression of genes for anaplerotic reactions in drying crusts, and the stable maintenance of mRNA through dormancy. These data shed light on possible synchronization between this cyanobacterium and its environment, and provides key mechanistic insights into its metabolism in situ that may be used to predict its response to climate, and or, land-use driven perturbations.

Original languageEnglish (US)
Pages (from-to)2178-2191
Number of pages14
JournalISME Journal
Volume7
Issue number11
DOIs
StatePublished - Nov 2013

Fingerprint

Desiccation
soil crusts
soil crust
dehydration (animal physiology)
desiccation (plant physiology)
dynamic response
Dehydration
hydration
dehydration
deserts
Soil
desert
Cyanobacteria
dormancy
desiccation
gene
stress response
Microcoleus vaginatus
drying
Osmoregulation

Keywords

  • biological soil crust
  • desiccation survival
  • dormancy
  • Microcoleus vaginatus
  • pulsed-activity event
  • resuscitation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

Rajeev, L., Da Rocha, U. N., Klitgord, N., Luning, E. G., Fortney, J., Axen, S. D., ... Mukhopadhyay, A. (2013). Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust. ISME Journal, 7(11), 2178-2191. https://doi.org/10.1038/ismej.2013.83

Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust. / Rajeev, Lara; Da Rocha, Ulisses Nunes; Klitgord, Niels; Luning, Eric G.; Fortney, Julian; Axen, Seth D.; Shih, Patrick M.; Bouskill, Nicholas J.; Bowen, Benjamin P.; Kerfeld, Cheryl A.; Garcia-Pichel, Ferran; Brodie, Eoin L.; Northen, Trent R.; Mukhopadhyay, Aindrila.

In: ISME Journal, Vol. 7, No. 11, 11.2013, p. 2178-2191.

Research output: Contribution to journalArticle

Rajeev, L, Da Rocha, UN, Klitgord, N, Luning, EG, Fortney, J, Axen, SD, Shih, PM, Bouskill, NJ, Bowen, BP, Kerfeld, CA, Garcia-Pichel, F, Brodie, EL, Northen, TR & Mukhopadhyay, A 2013, 'Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust', ISME Journal, vol. 7, no. 11, pp. 2178-2191. https://doi.org/10.1038/ismej.2013.83
Rajeev L, Da Rocha UN, Klitgord N, Luning EG, Fortney J, Axen SD et al. Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust. ISME Journal. 2013 Nov;7(11):2178-2191. https://doi.org/10.1038/ismej.2013.83
Rajeev, Lara ; Da Rocha, Ulisses Nunes ; Klitgord, Niels ; Luning, Eric G. ; Fortney, Julian ; Axen, Seth D. ; Shih, Patrick M. ; Bouskill, Nicholas J. ; Bowen, Benjamin P. ; Kerfeld, Cheryl A. ; Garcia-Pichel, Ferran ; Brodie, Eoin L. ; Northen, Trent R. ; Mukhopadhyay, Aindrila. / Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust. In: ISME Journal. 2013 ; Vol. 7, No. 11. pp. 2178-2191.
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