Dually fluorescent core-shell microgels for ratiometric imaging in live antigen-presenting cells

Xianfeng Zhou, Fengyu Su, Yanqing Tian, Deirdre Meldrum

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Core-shell microgels containing sensors/dyes in a matrix were fabricated by two-stage free radical precipitation polymerization method for ratiometric sensing/imaging. The microgels composing of poly(N-isopropylacrylamide) (PNIPAm) shell exhibits a low critical solution temperature (LCST), underwent an entropically driven transition from a swollen state to a deswollen state, which exhibit a hydrodynamic radius of ∼450 nm at 25uC (in vitro) and ∼190 nm at 37°C (in vivo). The microgel's ability of escaping from lysosome into cytosol makes the microgel be a potential candidate for cytosolic delivery of sensors/probes. Non-invasive imaging/sensing in Antigen-presenting cells (APCs) was feasible by monitoring the changes of fluorescence intensity ratios. Thus, these biocompatible microgels-based imaging/sensing agents may be expected to expand current molecular imaging/sensing techniques into methods applicable to studies in vivo, which could further drive APC-based treatments.

Original languageEnglish (US)
Article numbere88185
JournalPLoS One
Volume9
Issue number2
DOIs
StatePublished - Feb 4 2014

Fingerprint

antigen-presenting cells
Antigen-Presenting Cells
image analysis
Imaging techniques
Molecular Imaging
Hydrodynamics
Lysosomes
Polymerization
Cytosol
Free Radicals
Molecular imaging
Coloring Agents
Fluorescence
Sensors
Temperature
lysosomes
in vivo studies
cytosol
polymerization
hydrodynamics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Dually fluorescent core-shell microgels for ratiometric imaging in live antigen-presenting cells. / Zhou, Xianfeng; Su, Fengyu; Tian, Yanqing; Meldrum, Deirdre.

In: PLoS One, Vol. 9, No. 2, e88185, 04.02.2014.

Research output: Contribution to journalArticle

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