Droplet triggering for serial femtosecond crystallography using 3d-printed microfluidics

Daihyun Kim, Austin Echelmeier, Jorvani Cruz Villarreal, Sahir Gandhi, Sebastian Quintana, Ana Egatz-Gomez, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Structural biology at X-ray free-electron lasers (XFELs) is a compelling new technique. The majority of protein crystal sample, however, is wasted when continuously injected into the XFEL beam. Here, we present a new advancement in microfluidics by delivering crystal sample in droplets intersected by an oil phase with electrical control over the droplet generation parameters. Using integrated electrodes in a 3D-printed device, we demonstrate droplet generation, control of droplet frequency, and introduction of a phase shift for synchronization with the XFEL. This method has the potential to reduce sample waste for structural biology with XFELs by > 95%.

Original languageEnglish (US)
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1470-1471
Number of pages2
ISBN (Electronic)9781733419000
StatePublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: Oct 27 2019Oct 31 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period10/27/1910/31/19

Keywords

  • 3D-printing
  • Droplet microfluidics
  • Electrowetting
  • Serial Femtosecond Crystallography

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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