Droplet streams for serial crystallography of proteins

Uwe Weierstall; R. B. Doak; John Spence; D. Starodub; D. Shapiro; P. Kennedy; J. Warner; G. G. Hembree; Petra Fromme; H. N. Chapman

doi:10.1007/s00348-007-0426-8

Droplet streams for serial crystallography of proteins

Uwe Weierstall, R. B. Doak, John Spence, D. Starodub, D. Shapiro, P. Kennedy, J. Warner, G. G. Hembree, Petra Fromme, H. N. Chapman

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Serial diffraction of proteins requires an injection method to deliver analyte molecules-preferably uncharged, fully hydrated, spatially oriented, and with high flux-into a focused probe beam of electrons or X-rays that is only a few tens of microns in diameter. This work examines conventional Rayleigh sources and electrospray-assisted Rayleigh sources as to their suitability for this task. A comparison is made and conclusions drawn on the basis of time-resolved optical images of the droplet streams produced by these sources. Straight-line periodic streams of monodisperse droplets were generated with both sources, achieving droplet diameters of 4 and 1 micrometer, respectively, for the conventional and electrospray-assisted versions. Shrinkage of droplets by evaporation is discussed and quantified. It is shown experimentally that proteins pass undamaged through a conventional Rayleigh droplet source.

Original language	English (US)
Pages (from-to)	675-689
Number of pages	15
Journal	Experiments in Fluids
Volume	44
Issue number	5
DOIs	https://doi.org/10.1007/s00348-007-0426-8
State	Published - May 2008

ASJC Scopus subject areas

Computational Mechanics
Mechanics of Materials
Physics and Astronomy(all)
Fluid Flow and Transfer Processes

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10.1007/s00348-007-0426-8

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abstract = "Serial diffraction of proteins requires an injection method to deliver analyte molecules-preferably uncharged, fully hydrated, spatially oriented, and with high flux-into a focused probe beam of electrons or X-rays that is only a few tens of microns in diameter. This work examines conventional Rayleigh sources and electrospray-assisted Rayleigh sources as to their suitability for this task. A comparison is made and conclusions drawn on the basis of time-resolved optical images of the droplet streams produced by these sources. Straight-line periodic streams of monodisperse droplets were generated with both sources, achieving droplet diameters of 4 and 1 micrometer, respectively, for the conventional and electrospray-assisted versions. Shrinkage of droplets by evaporation is discussed and quantified. It is shown experimentally that proteins pass undamaged through a conventional Rayleigh droplet source.",

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T1 - Droplet streams for serial crystallography of proteins

AU - Weierstall, Uwe

AU - Doak, R. B.

AU - Spence, John

AU - Starodub, D.

AU - Shapiro, D.

AU - Kennedy, P.

AU - Warner, J.

AU - Hembree, G. G.

AU - Fromme, Petra

AU - Chapman, H. N.

PY - 2008/5

Y1 - 2008/5

N2 - Serial diffraction of proteins requires an injection method to deliver analyte molecules-preferably uncharged, fully hydrated, spatially oriented, and with high flux-into a focused probe beam of electrons or X-rays that is only a few tens of microns in diameter. This work examines conventional Rayleigh sources and electrospray-assisted Rayleigh sources as to their suitability for this task. A comparison is made and conclusions drawn on the basis of time-resolved optical images of the droplet streams produced by these sources. Straight-line periodic streams of monodisperse droplets were generated with both sources, achieving droplet diameters of 4 and 1 micrometer, respectively, for the conventional and electrospray-assisted versions. Shrinkage of droplets by evaporation is discussed and quantified. It is shown experimentally that proteins pass undamaged through a conventional Rayleigh droplet source.

AB - Serial diffraction of proteins requires an injection method to deliver analyte molecules-preferably uncharged, fully hydrated, spatially oriented, and with high flux-into a focused probe beam of electrons or X-rays that is only a few tens of microns in diameter. This work examines conventional Rayleigh sources and electrospray-assisted Rayleigh sources as to their suitability for this task. A comparison is made and conclusions drawn on the basis of time-resolved optical images of the droplet streams produced by these sources. Straight-line periodic streams of monodisperse droplets were generated with both sources, achieving droplet diameters of 4 and 1 micrometer, respectively, for the conventional and electrospray-assisted versions. Shrinkage of droplets by evaporation is discussed and quantified. It is shown experimentally that proteins pass undamaged through a conventional Rayleigh droplet source.

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Droplet streams for serial crystallography of proteins

Abstract

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