Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)

Karyna Rosario, Milen Marinov, Daisy Stainton, Simona Kraberger, Elizabeth J. Wiltshire, David A. Collings, Matthew Walters, Darren P. Martin, Mya Breitbart, Arvind Varsani

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share > 95% nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48-63% genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.

Original languageEnglish (US)
Pages (from-to)1302-1308
Number of pages7
JournalJournal of General Virology
Volume92
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

Fingerprint

Odonata
DNA Viruses
Single-Stranded DNA
Circoviridae
Genome
Metagenomics
Viruses
Genetic Recombination
Tonga
Nucleotides
Insects
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology

Cite this

Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata : Anisoptera). / Rosario, Karyna; Marinov, Milen; Stainton, Daisy; Kraberger, Simona; Wiltshire, Elizabeth J.; Collings, David A.; Walters, Matthew; Martin, Darren P.; Breitbart, Mya; Varsani, Arvind.

In: Journal of General Virology, Vol. 92, No. 6, 06.2011, p. 1302-1308.

Research output: Contribution to journalArticle

Rosario, K, Marinov, M, Stainton, D, Kraberger, S, Wiltshire, EJ, Collings, DA, Walters, M, Martin, DP, Breitbart, M & Varsani, A 2011, 'Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)', Journal of General Virology, vol. 92, no. 6, pp. 1302-1308. https://doi.org/10.1099/vir.0.030338-0
Rosario, Karyna ; Marinov, Milen ; Stainton, Daisy ; Kraberger, Simona ; Wiltshire, Elizabeth J. ; Collings, David A. ; Walters, Matthew ; Martin, Darren P. ; Breitbart, Mya ; Varsani, Arvind. / Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata : Anisoptera). In: Journal of General Virology. 2011 ; Vol. 92, No. 6. pp. 1302-1308.
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