Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)

Karyna Rosario; Milen Marinov; Daisy Stainton; Simona Kraberger; Elizabeth J. Wiltshire; David A. Collings; Matthew Walters; Darren P. Martin; Mya Breitbart; Arvind Varsani

doi:10.1099/vir.0.030338-0

Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)

Karyna Rosario, Milen Marinov, Daisy Stainton, Simona Kraberger, Elizabeth J. Wiltshire, David A. Collings, Matthew Walters, Darren P. Martin, Mya Breitbart, Arvind Varsani

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share > 95% nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48-63% genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.

Original language	English (US)
Pages (from-to)	1302-1308
Number of pages	7
Journal	Journal of General Virology
Volume	92
Issue number	6
DOIs	https://doi.org/10.1099/vir.0.030338-0
State	Published - Jun 2011
Externally published	Yes

ASJC Scopus subject areas

Virology

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title = "Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)",

abstract = "Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share > 95% nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48-63% genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.",

author = "Karyna Rosario and Milen Marinov and Daisy Stainton and Simona Kraberger and Wiltshire, {Elizabeth J.} and Collings, {David A.} and Matthew Walters and Martin, {Darren P.} and Mya Breitbart and Arvind Varsani",

year = "2011",

month = jun,

doi = "10.1099/vir.0.030338-0",

language = "English (US)",

volume = "92",

pages = "1302--1308",

journal = "Journal of General Virology",

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publisher = "Society for General Microbiology",

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T2 - Anisoptera)

AU - Rosario, Karyna

AU - Marinov, Milen

AU - Stainton, Daisy

AU - Kraberger, Simona

AU - Wiltshire, Elizabeth J.

AU - Collings, David A.

AU - Walters, Matthew

AU - Martin, Darren P.

AU - Breitbart, Mya

AU - Varsani, Arvind

PY - 2011/6

Y1 - 2011/6

N2 - Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share > 95% nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48-63% genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.

AB - Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share > 95% nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48-63% genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.

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Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)

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