Down-regulation of honey bee IRS gene biases behavior toward food rich in protein

Ying Wang, Navdeep S. Mutti, Kate E. Ihle, Adam Siegel, Adam G. Dolezal, Osman Kaftanoglu, Gro Amdam

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Food choice and eating behavior affect health and longevity. Large-scale research efforts aim to understand the molecular and social/behavioral mechanisms of energy homeostasis, body weight, and food intake. Honey bees (Apis mellifera) could provide a model for these studies since individuals vary in food-related behavior and social factors can be controlled. Here, we examine a potential role of peripheral insulin receptor substrate (IRS) expression in honey bee foraging behavior. IRS is central to cellular nutrient sensing through transduction of insulin/insulin-like signals (IIS). By reducing peripheral IRS gene expression and IRS protein amount with the use of RNA interference (RNAi), we demonstrate that IRS influences foraging choice in two standard strains selected for different food-hoarding behavior. Compared with controls, IRS knockdowns bias their foraging effort toward protein (pollen) rather than toward carbohydrate (nectar) sources. Through control experiments, we establish that IRS does not influence the bees' sucrose sensory response, a modality that is generally associated with food-related behavior and specifically correlated with the foraging preference of honey bees. These results reveal a new affector pathway of honey bee social foraging, and suggest that IRS expressed in peripheral tissue can modulate an insect's foraging choice between protein and carbohydrate sources.

Original languageEnglish (US)
JournalPLoS Genetics
Volume6
Issue number4
DOIs
StatePublished - Apr 2010

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Honey
Bees
Insulin Receptor
honey
bee
honey bees
Down-Regulation
substrate
Food
protein
food
gene
foraging
Genes
Proteins
genes
proteins
carbohydrate
Carbohydrates
Insulin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Down-regulation of honey bee IRS gene biases behavior toward food rich in protein. / Wang, Ying; Mutti, Navdeep S.; Ihle, Kate E.; Siegel, Adam; Dolezal, Adam G.; Kaftanoglu, Osman; Amdam, Gro.

In: PLoS Genetics, Vol. 6, No. 4, 04.2010.

Research output: Contribution to journalArticle

Wang, Ying ; Mutti, Navdeep S. ; Ihle, Kate E. ; Siegel, Adam ; Dolezal, Adam G. ; Kaftanoglu, Osman ; Amdam, Gro. / Down-regulation of honey bee IRS gene biases behavior toward food rich in protein. In: PLoS Genetics. 2010 ; Vol. 6, No. 4.
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