Double-focusing mixing jet for XFEL study of chemical kinetics

Dingjie Wang, Uwe Weierstall, Lois Pollack, John Spence

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    Several liquid sample injection methods have been developed to satisfy the requirements for serial femtosecond X-ray nanocrystallography, which enables radiation-damage-free determination of molecular structure at room temperature. Time-resolved nanocrystallography would combine structure analysis with chemical kinetics by determining the structures of the transient states and chemical kinetic mechanisms simultaneously. A windowless liquid mixing jet device has been designed for this purpose. It achieves fast uniform mixing of substrates and enzymes in the jet within 250 µs, with an adjustable delay between mixing and probing by the X-ray free-electron laser beam of up to 1 s for each frame of a `movie'. The principle of the liquid mixing jet device is illustrated using numerical simulation, and experimental results are presented using a fluorescent dye.

    Original languageEnglish (US)
    Pages (from-to)1364-1366
    Number of pages3
    JournalJournal of Synchrotron Radiation
    Volume21
    DOIs
    StatePublished - Nov 1 2014

    Fingerprint

    Reaction kinetics
    reaction kinetics
    X-Rays
    Equipment and Supplies
    Motion Pictures
    Molecular Structure
    Fluorescent Dyes
    Liquids
    Lasers
    liquids
    Electrons
    Radiation
    X ray lasers
    Injections
    Temperature
    Free electron lasers
    Radiation damage
    Enzymes
    radiation damage
    free electron lasers

    Keywords

    • chemical kinetics
    • mixing jet
    • sample injection
    • time-resolved diffraction

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Double-focusing mixing jet for XFEL study of chemical kinetics. / Wang, Dingjie; Weierstall, Uwe; Pollack, Lois; Spence, John.

    In: Journal of Synchrotron Radiation, Vol. 21, 01.11.2014, p. 1364-1366.

    Research output: Contribution to journalArticle

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