DNA polymerase-mediated DNA synthesis on a TNA template

John C. Chaput; Justin K. Ichida; Jack W. Szostak

doi:10.1021/ja028589k

DNA polymerase-mediated DNA synthesis on a TNA template

John C. Chaput, Justin K. Ichida, Jack W. Szostak

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.

Original language	English (US)
Pages (from-to)	856-857
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	4
DOIs	https://doi.org/10.1021/ja028589k
State	Published - Jan 29 2003
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja028589k

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DNA polymerase-mediated DNA synthesis on a TNA template

Abstract

ASJC Scopus subject areas

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