DNA polymerase β lyase inhibitors from Maytenus putterlickoides

Xizhi Feng, Zhijie Gao, Shisheng Li, Shannon H. Jones, Sidney M. Hecht

Research output: Contribution to journalReview article

16 Scopus citations

Abstract

During a survey of plant secondary metabolites for DNA polymerase β lyase inhibitors, we found that a crude methyl ethyl ketone extract prepared from Maytenus putterlickoides showed strong inhibition of the lyase activity of DNA polymerase β in an in vitro assay. Bioassay-guided fractionation of the extract, using an in vitro assay, resulted in the discovery of a new active principle, 30-(4′-hydroxybenzoyloxy)-11α-hydroxylupane-20(29)-en-3- one (1), as well as a known compound, (-)-epicatechin (2). Compounds 1 and 2 exhibited DNA polymerase β lyase inhibitory activity with IC50 values of 62.8 and 18.5 μM, respectively. Compound 2 was capable of potentiating the action of the monofunctional methylating agent methyl methanesulfonate in cultured human cancer cells, consistent with the possible utility of inhibitors of this type in vivo.

Original languageEnglish (US)
Pages (from-to)1744-1747
Number of pages4
JournalJournal of Natural Products
Volume67
Issue number10
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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