Diversity and evolution of the envelope gene of dengue virus type 1

Ana P. Goncalvez, Ananias A. Escalante, Flor H. Pujol, Juan E. Ludert, Duilia Tovar, Rosa A. Salas, Ferdinando Liprandi

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The genetic diversity and phylogenetic relationships of a collection of strains of dengue virus type 1 (DV-1), isolated from different parts of the world, were investigated. Phylogenetic trees derived from the complete sequence of the E gene of 44 strains suggested the existence of five genetic types defined by a maximum nucleotide divergence within each group of 6%. The 22 strains from America were classified into a single genetic type that included strains associated either with classical dengue or hemorrhagic dengue episodes. Using a maximum likelihood procedure based on a single rate with dated tips model and substitution rates calculated at the third codon position, evolution of the five DV-1 genotypes was shown to conform to a molecular clock. The average rate of evolution was estimated to be approximately 16.2 × 104 substitutions/third codon position site/year. Using this estimate, divergence among the DV-1 genotypes was calculated to have occurred approximately 100 years ago. Very low average value of the ratio of nonsynonymous-to-synonymous nucleotide substitutions, relative to the respective sites (0.046), indicated that the evolution of the E gene of the DV-1 is subject mostly to purifying selection.

Original languageEnglish (US)
Pages (from-to)110-119
Number of pages10
JournalVirology
Volume303
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

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Dengue Virus
Codon
Genes
Nucleotides
Genotype
Severe Dengue
Dengue

Keywords

  • Dengue
  • Dengue virus
  • Envelope
  • Genetic diversity
  • Molecular clock
  • Molecular epidemiology
  • Molecular evolution
  • Nucleotide substitutions

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Goncalvez, A. P., Escalante, A. A., Pujol, F. H., Ludert, J. E., Tovar, D., Salas, R. A., & Liprandi, F. (2002). Diversity and evolution of the envelope gene of dengue virus type 1. Virology, 303(1), 110-119. https://doi.org/10.1006/viro.2002.1686

Diversity and evolution of the envelope gene of dengue virus type 1. / Goncalvez, Ana P.; Escalante, Ananias A.; Pujol, Flor H.; Ludert, Juan E.; Tovar, Duilia; Salas, Rosa A.; Liprandi, Ferdinando.

In: Virology, Vol. 303, No. 1, 2002, p. 110-119.

Research output: Contribution to journalArticle

Goncalvez, AP, Escalante, AA, Pujol, FH, Ludert, JE, Tovar, D, Salas, RA & Liprandi, F 2002, 'Diversity and evolution of the envelope gene of dengue virus type 1', Virology, vol. 303, no. 1, pp. 110-119. https://doi.org/10.1006/viro.2002.1686
Goncalvez AP, Escalante AA, Pujol FH, Ludert JE, Tovar D, Salas RA et al. Diversity and evolution of the envelope gene of dengue virus type 1. Virology. 2002;303(1):110-119. https://doi.org/10.1006/viro.2002.1686
Goncalvez, Ana P. ; Escalante, Ananias A. ; Pujol, Flor H. ; Ludert, Juan E. ; Tovar, Duilia ; Salas, Rosa A. ; Liprandi, Ferdinando. / Diversity and evolution of the envelope gene of dengue virus type 1. In: Virology. 2002 ; Vol. 303, No. 1. pp. 110-119.
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