TY - JOUR
T1 - Diverse single‐stranded dna viruses identified in chicken buccal swabs
AU - Chrzastek, Klaudia
AU - Kraberger, Simona
AU - Schmidlin, Kara
AU - Fontenele, Rafaela S.
AU - Kulkarni, Arun
AU - Chappell, Len
AU - Dufour‐zavala, Louise
AU - Kapczynski, Darrell R.
AU - Varsani, Arvind
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - High‐throughput sequencing approaches offer the possibility to better understand the complex microbial communities associated with animals. Viral metagenomics has facilitated the discovery and identification of many known and unknown viruses that inhabit mucosal surfaces of the body and has extended our knowledge related to virus diversity. We used metagenomics sequencing of chicken buccal swab samples and identified various small DNA viruses with circular genome organization. Out of 134 putative circular viral‐like circular genome sequences, 70 are cress-dnaviruses and 26 are microviruses, whilst the remaining 38 most probably represent sub‐genomic molecules. The cressdnaviruses found in this study belong to the Circoviridae, Genomoviridae and Smacoviridae families as well as previously described CRESS1 and naryavirus groups. Among these, genomoviruses and smacoviruses were the most prevalent across the samples. Interestingly, we also identified 26 bacteriophages that belong to the Microviridae family, whose members are known to infect enterobacteria.
AB - High‐throughput sequencing approaches offer the possibility to better understand the complex microbial communities associated with animals. Viral metagenomics has facilitated the discovery and identification of many known and unknown viruses that inhabit mucosal surfaces of the body and has extended our knowledge related to virus diversity. We used metagenomics sequencing of chicken buccal swab samples and identified various small DNA viruses with circular genome organization. Out of 134 putative circular viral‐like circular genome sequences, 70 are cress-dnaviruses and 26 are microviruses, whilst the remaining 38 most probably represent sub‐genomic molecules. The cressdnaviruses found in this study belong to the Circoviridae, Genomoviridae and Smacoviridae families as well as previously described CRESS1 and naryavirus groups. Among these, genomoviruses and smacoviruses were the most prevalent across the samples. Interestingly, we also identified 26 bacteriophages that belong to the Microviridae family, whose members are known to infect enterobacteria.
KW - Chicken
KW - Cressdnaviricota
KW - Microviridae
KW - Respiratory tract
KW - Single‐stranded DNA viruses
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U2 - 10.3390/microorganisms9122602
DO - 10.3390/microorganisms9122602
M3 - Article
AN - SCOPUS:85121122314
VL - 9
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 12
M1 - 2602
ER -