TY - JOUR
T1 - Diverse circular replication-associated protein encoding viruses circulating in invertebrates within a lake ecosystem
AU - Dayaram, Anisha
AU - Galatowitsch, Mark L.
AU - Argüello-Astorga, Gerardo R.
AU - van Bysterveldt, Katherine
AU - Kraberger, Simona
AU - Stainton, Daisy
AU - Harding, Jon S.
AU - Roumagnac, Philippe
AU - Martin, Darren P.
AU - Lefeuvre, Pierre
AU - Varsani, Arvind
N1 - Funding Information:
This work was funded by a grant awarded to AD, MG, JH and AV from the Brian Mason Scientific and Technical Trust (New Zealand) . We thank University of Canterbury for the use of the Cass field station. AV and DPM are supported by the National Research Foundation of South Africa ( 2012/5 ).
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Over the last five years next-generation sequencing has become a cost effective and efficient method for identifying known and unknown microorganisms. Access to this technique has dramatically changed the field of virology, enabling a wide range of environmental viral metagenome studies to be undertaken of organisms and environmental samples from polar to tropical regions. These studies have led to the discovery of hundreds of highly divergent single stranded DNA (ssDNA) virus-like sequences encoding replication-associated proteins. Yet, few studies have explored how viruses might be shared in an ecosystem through feeding relationships. Here we identify 169 circular molecules (160 CRESS DNA molecules, nine circular molecules) recovered from a New Zealand freshwater lake, that we have tentatively classified into 51 putatively novel species and five previously described species (DflaCV-3, -5, -6, -8, -10). The CRESS DNA viruses identified in this study were recovered from molluscs (Echyridella menzeisii, Musculium novaezelandiae, Potamopyrgus antipodarum and Physella acuta) and insect larvae (Procordulia grayi, Xanthocnemis zealandica, and Chironomus zealandicus) collected from Lake Sarah, as well as from the lake water and benthic sediments. Extensive diversity was observed across most CRESS DNA molecules recovered. The putative capsid protein of one viral species was found to be most similar to those of members of the Tombusviridae family, thus expanding the number of known RNA-DNA hybrid viruses in nature. We noted a strong association between the CRESS DNA viruses and circular molecules identified in the water and browser organisms (C. zealandicus, P. antipodarum and P. acuta), and between water sediments and undefended prey species (C. zealandicus). However, we were unable to find any significant correlation of viral assemblages to the potential feeding relationships of the host aquatic invertebrates.
AB - Over the last five years next-generation sequencing has become a cost effective and efficient method for identifying known and unknown microorganisms. Access to this technique has dramatically changed the field of virology, enabling a wide range of environmental viral metagenome studies to be undertaken of organisms and environmental samples from polar to tropical regions. These studies have led to the discovery of hundreds of highly divergent single stranded DNA (ssDNA) virus-like sequences encoding replication-associated proteins. Yet, few studies have explored how viruses might be shared in an ecosystem through feeding relationships. Here we identify 169 circular molecules (160 CRESS DNA molecules, nine circular molecules) recovered from a New Zealand freshwater lake, that we have tentatively classified into 51 putatively novel species and five previously described species (DflaCV-3, -5, -6, -8, -10). The CRESS DNA viruses identified in this study were recovered from molluscs (Echyridella menzeisii, Musculium novaezelandiae, Potamopyrgus antipodarum and Physella acuta) and insect larvae (Procordulia grayi, Xanthocnemis zealandica, and Chironomus zealandicus) collected from Lake Sarah, as well as from the lake water and benthic sediments. Extensive diversity was observed across most CRESS DNA molecules recovered. The putative capsid protein of one viral species was found to be most similar to those of members of the Tombusviridae family, thus expanding the number of known RNA-DNA hybrid viruses in nature. We noted a strong association between the CRESS DNA viruses and circular molecules identified in the water and browser organisms (C. zealandicus, P. antipodarum and P. acuta), and between water sediments and undefended prey species (C. zealandicus). However, we were unable to find any significant correlation of viral assemblages to the potential feeding relationships of the host aquatic invertebrates.
KW - CRESS DNA viruses
KW - Chironomus zealandicus
KW - Echyridella menzeisi
KW - Freshwater invertebrates
KW - Musculium novazelandiae
KW - Physella acuta
KW - Potamopyrgus antipodarum
KW - Procordulia grayi
KW - Replication-associated protein
KW - Xanthocnemis zealandica
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UR - http://www.scopus.com/inward/citedby.url?scp=84958247929&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2016.02.011
DO - 10.1016/j.meegid.2016.02.011
M3 - Article
C2 - 26873065
AN - SCOPUS:84958247929
VL - 39
SP - 304
EP - 316
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
ER -
