Divalent metal transporter, DMT1: A novel MRI reporter protein

Benjamin B. Bartelle, Kamila U. Szulc, Giselle A. Suero-Abreu, Joe J. Rodriguez, Daniel H. Turnbull

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Manganese (Mn)-enhanced MRI (MEMRI) has found a growing number of applications in anatomical and functional imaging in small animals, based on the cellular uptake of Mn ions in the brain, heart, and other organs. Previous studies have relied on endogenous mechanisms of paramagnetic Mn ion uptake and enhancement. To genetically control MEMRI signals, we reverse engineered a major component of the molecular machinery involved in Mn uptake, the divalent metal transporter, DMT1. DMT1 provides positive cellular enhancement in a manner that is highly sensitive and dynamic, allowing greater spatial and temporal resolution for MRI compared to previously proposed MRI reporters such as ferritin. We characterized the MEMRI signal enhancement properties of DMT1-expressing cells, both in vitro and in vivo in mouse models of cancer and brain development. Our results show that DMT1 provides an effective genetic MRI reporter for a wide range of biological and preclinical imaging applications.

Original languageEnglish (US)
Pages (from-to)842-850
Number of pages9
JournalMagnetic Resonance in Medicine
Volume70
Issue number3
DOIs
StatePublished - Sep 2013
Externally publishedYes

Keywords

  • Mn-enhanced MRI (MEMRI)
  • manganese (Mn)
  • molecular imaging
  • reporter protein

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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