Divalent metal transporter, DMT1: A novel MRI reporter protein

Benjamin B. Bartelle; Kamila U. Szulc; Giselle A. Suero-Abreu; Joe J. Rodriguez; Daniel H. Turnbull

doi:10.1002/mrm.24509

Divalent metal transporter, DMT1: A novel MRI reporter protein

Benjamin B. Bartelle, Kamila U. Szulc, Giselle A. Suero-Abreu, Joe J. Rodriguez, Daniel H. Turnbull

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Manganese (Mn)-enhanced MRI (MEMRI) has found a growing number of applications in anatomical and functional imaging in small animals, based on the cellular uptake of Mn ions in the brain, heart, and other organs. Previous studies have relied on endogenous mechanisms of paramagnetic Mn ion uptake and enhancement. To genetically control MEMRI signals, we reverse engineered a major component of the molecular machinery involved in Mn uptake, the divalent metal transporter, DMT1. DMT1 provides positive cellular enhancement in a manner that is highly sensitive and dynamic, allowing greater spatial and temporal resolution for MRI compared to previously proposed MRI reporters such as ferritin. We characterized the MEMRI signal enhancement properties of DMT1-expressing cells, both in vitro and in vivo in mouse models of cancer and brain development. Our results show that DMT1 provides an effective genetic MRI reporter for a wide range of biological and preclinical imaging applications.

Original language	English (US)
Pages (from-to)	842-850
Number of pages	9
Journal	Magnetic Resonance in Medicine
Volume	70
Issue number	3
DOIs	https://doi.org/10.1002/mrm.24509
State	Published - Sep 2013
Externally published	Yes

Keywords

Mn-enhanced MRI (MEMRI)
manganese (Mn)
molecular imaging
reporter protein

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Access to Document

10.1002/mrm.24509

Cite this

@article{d70a8931eedb48de89500f75da6ed56c,

title = "Divalent metal transporter, DMT1: A novel MRI reporter protein",

abstract = "Manganese (Mn)-enhanced MRI (MEMRI) has found a growing number of applications in anatomical and functional imaging in small animals, based on the cellular uptake of Mn ions in the brain, heart, and other organs. Previous studies have relied on endogenous mechanisms of paramagnetic Mn ion uptake and enhancement. To genetically control MEMRI signals, we reverse engineered a major component of the molecular machinery involved in Mn uptake, the divalent metal transporter, DMT1. DMT1 provides positive cellular enhancement in a manner that is highly sensitive and dynamic, allowing greater spatial and temporal resolution for MRI compared to previously proposed MRI reporters such as ferritin. We characterized the MEMRI signal enhancement properties of DMT1-expressing cells, both in vitro and in vivo in mouse models of cancer and brain development. Our results show that DMT1 provides an effective genetic MRI reporter for a wide range of biological and preclinical imaging applications.",

keywords = "Mn-enhanced MRI (MEMRI), manganese (Mn), molecular imaging, reporter protein",

author = "Bartelle, {Benjamin B.} and Szulc, {Kamila U.} and Suero-Abreu, {Giselle A.} and Rodriguez, {Joe J.} and Turnbull, {Daniel H.}",

year = "2013",

month = sep,

doi = "10.1002/mrm.24509",

language = "English (US)",

volume = "70",

pages = "842--850",

journal = "Magnetic Resonance in Medicine",

issn = "0740-3194",

publisher = "John Wiley and Sons Inc.",

number = "3",

}

TY - JOUR

T1 - Divalent metal transporter, DMT1

T2 - A novel MRI reporter protein

AU - Bartelle, Benjamin B.

AU - Szulc, Kamila U.

AU - Suero-Abreu, Giselle A.

AU - Rodriguez, Joe J.

AU - Turnbull, Daniel H.

PY - 2013/9

Y1 - 2013/9

N2 - Manganese (Mn)-enhanced MRI (MEMRI) has found a growing number of applications in anatomical and functional imaging in small animals, based on the cellular uptake of Mn ions in the brain, heart, and other organs. Previous studies have relied on endogenous mechanisms of paramagnetic Mn ion uptake and enhancement. To genetically control MEMRI signals, we reverse engineered a major component of the molecular machinery involved in Mn uptake, the divalent metal transporter, DMT1. DMT1 provides positive cellular enhancement in a manner that is highly sensitive and dynamic, allowing greater spatial and temporal resolution for MRI compared to previously proposed MRI reporters such as ferritin. We characterized the MEMRI signal enhancement properties of DMT1-expressing cells, both in vitro and in vivo in mouse models of cancer and brain development. Our results show that DMT1 provides an effective genetic MRI reporter for a wide range of biological and preclinical imaging applications.

AB - Manganese (Mn)-enhanced MRI (MEMRI) has found a growing number of applications in anatomical and functional imaging in small animals, based on the cellular uptake of Mn ions in the brain, heart, and other organs. Previous studies have relied on endogenous mechanisms of paramagnetic Mn ion uptake and enhancement. To genetically control MEMRI signals, we reverse engineered a major component of the molecular machinery involved in Mn uptake, the divalent metal transporter, DMT1. DMT1 provides positive cellular enhancement in a manner that is highly sensitive and dynamic, allowing greater spatial and temporal resolution for MRI compared to previously proposed MRI reporters such as ferritin. We characterized the MEMRI signal enhancement properties of DMT1-expressing cells, both in vitro and in vivo in mouse models of cancer and brain development. Our results show that DMT1 provides an effective genetic MRI reporter for a wide range of biological and preclinical imaging applications.

KW - Mn-enhanced MRI (MEMRI)

KW - manganese (Mn)

KW - molecular imaging

KW - reporter protein

UR - http://www.scopus.com/inward/record.url?scp=84883219025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883219025&partnerID=8YFLogxK

U2 - 10.1002/mrm.24509

DO - 10.1002/mrm.24509

M3 - Article

C2 - 23065715

AN - SCOPUS:84883219025

SN - 0740-3194

VL - 70

SP - 842

EP - 850

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

IS - 3

ER -

Divalent metal transporter, DMT1: A novel MRI reporter protein

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this