Disruption of microtubules ablates the specificity of insulin signaling to GLUT4 translocation in 3T3-L1 adipocytes

Jie Huang, Takeshi Imamura, Jennie Bever, Juu Chin Lu, Jerrold M. Olefsky

Research output: Contribution to journalArticle

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Abstract

Although the cytoskeletal network is important for insulin-induced glucose uptake, several studies have assessed the effects of microtubule disruption on glucose transport with divergent results. Here, we investigated the effects of microtubule-depolymerizing reagent, nocodazole and colchicine, on GLUT4 translocation in 3T3-L1 adipocytes. After nocodazole treatment to disrupt microtubules, GLUT4 vesicles were dispersed from the perinuclear region in the basal state, and insulin-induced GLUT4 translocation was partially inhibited by 20-30%, consistent with other reports. We found that platelet-derived growth factor (PDGF), which did not stimulate GLUT4 translocation in intact cells, was surprisingly able to enhance GLUT4 translocation to -50% of the maximal insulin response, in nocodazole-treated cells with disrupted microtubules. This effect of PDGF was blocked by pretreatment with wortmannin and attenuated in cells pretreated with cytochalasin D. Using confocal microscopy, we found an increased co-localization of GLUT4 and F-actin in nocodazole-treated cells upon PDGF stimulation compared with control cells. Furthermore, microinjection of small interfering RNA targeting the actin-based motor Myolc, but not the microtubule-based motor KIF3, significantly inhibited both insulin- and PDGF-stimulated GLUT4 translocation after nocodazole treatment. In summary, our data suggest that 1) proper perinuclear localization of GLUT4 vesicles is a requirement for insulin-specific stimulation of GLUT4 translocation, and 2) nocodazole treatment disperses GLUT4 vesicles from the perinuclear region allowing them to engage insulin and PDGF-sensitive actin filaments, which can participate in GLUT4 translocation in a phosphatidylinositol 3-kinase-dependent manner.

Original languageEnglish (US)
Pages (from-to)42300-42306
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number51
DOIs
StatePublished - Dec 23 2005
Externally publishedYes

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Nocodazole
Adipocytes
Microtubules
Platelet-Derived Growth Factor
Insulin
Actins
Cells
Phosphatidylinositol 3-Kinase
Glucose
Cytochalasin D
Confocal microscopy
Colchicine
Microinjections
Actin Cytoskeleton
Confocal Microscopy
Small Interfering RNA
Therapeutics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Disruption of microtubules ablates the specificity of insulin signaling to GLUT4 translocation in 3T3-L1 adipocytes. / Huang, Jie; Imamura, Takeshi; Bever, Jennie; Lu, Juu Chin; Olefsky, Jerrold M.

In: Journal of Biological Chemistry, Vol. 280, No. 51, 23.12.2005, p. 42300-42306.

Research output: Contribution to journalArticle

Huang, Jie ; Imamura, Takeshi ; Bever, Jennie ; Lu, Juu Chin ; Olefsky, Jerrold M. / Disruption of microtubules ablates the specificity of insulin signaling to GLUT4 translocation in 3T3-L1 adipocytes. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 51. pp. 42300-42306.
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