TY - JOUR
T1 - Disposable ultrasound-sensing chronic cranial window by soft nanoimprinting lithography
AU - Li, Hao
AU - Dong, Biqin
AU - Zhang, Xian
AU - Shu, Xiao
AU - Chen, Xiangfan
AU - Hai, Rihan
AU - Czaplewski, David A.
AU - Zhang, Hao F.
AU - Sun, Cheng
N1 - Funding Information:
We thank Dr. Leonidas Ocola for his assistance in electron-beam nanolithography, and Dr. Ayush Batra and Dr. Neil Avadhoot Nadkarni for insightful advice and discussion on the hemorrhage and neovascularization observed during the long-term in vivo studies. We also thank Drs. Rui Cao and Song Hu for their assistance in mouse imaging. This work is supported by National Science Foundation grants DBI-1353952 and EEC-1530734; National Institutes of Health grants R01EY026078, R01EY029121, R01HL141933, and F30EY026472; a Research Catalyst Award by Northwestern McCormick School of Engineering, and a Northwestern University Innovative Initiative Incubator (I3) Award. Use of the Center for Nanoscale Materials, an Office of Science user facility, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02–06CH11357.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Chronic cranial window (CCW) is an essential tool in enabling longitudinal imaging and manipulation of various brain activities in live animals. However, an active CCW capable of sensing the concealed in vivo environment while simultaneously providing longitudinal optical access to the brain is not currently available. Here we report a disposable ultrasound-sensing CCW (usCCW) featuring an integrated transparent nanophotonic ultrasonic detector fabricated using soft nanoimprint lithography process. We optimize the sensor design and the associated fabrication process to significantly improve detection sensitivity and reliability, which are critical for the intend longitudinal in vivo investigations. Surgically implanting the usCCW on the skull creates a self-contained environment, maintaining optical access while eliminating the need for external ultrasound coupling medium for photoacoustic imaging. Using this usCCW, we demonstrate photoacoustic microscopy of cortical vascular network in live mice over 28 days. This work establishes the foundation for integrating photoacoustic imaging with modern brain research.
AB - Chronic cranial window (CCW) is an essential tool in enabling longitudinal imaging and manipulation of various brain activities in live animals. However, an active CCW capable of sensing the concealed in vivo environment while simultaneously providing longitudinal optical access to the brain is not currently available. Here we report a disposable ultrasound-sensing CCW (usCCW) featuring an integrated transparent nanophotonic ultrasonic detector fabricated using soft nanoimprint lithography process. We optimize the sensor design and the associated fabrication process to significantly improve detection sensitivity and reliability, which are critical for the intend longitudinal in vivo investigations. Surgically implanting the usCCW on the skull creates a self-contained environment, maintaining optical access while eliminating the need for external ultrasound coupling medium for photoacoustic imaging. Using this usCCW, we demonstrate photoacoustic microscopy of cortical vascular network in live mice over 28 days. This work establishes the foundation for integrating photoacoustic imaging with modern brain research.
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U2 - 10.1038/s41467-019-12178-6
DO - 10.1038/s41467-019-12178-6
M3 - Article
C2 - 31537800
AN - SCOPUS:85072404115
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 4277
ER -