Disposable ultrasound-sensing chronic cranial window by soft nanoimprinting lithography

Hao Li, Biqin Dong, Xian Zhang, Xiao Shu, Xiangfan Chen, Rihan Hai, David A. Czaplewski, Hao F. Zhang, Cheng Sun

Research output: Contribution to journalArticle

Abstract

Chronic cranial window (CCW) is an essential tool in enabling longitudinal imaging and manipulation of various brain activities in live animals. However, an active CCW capable of sensing the concealed in vivo environment while simultaneously providing longitudinal optical access to the brain is not currently available. Here we report a disposable ultrasound-sensing CCW (usCCW) featuring an integrated transparent nanophotonic ultrasonic detector fabricated using soft nanoimprint lithography process. We optimize the sensor design and the associated fabrication process to significantly improve detection sensitivity and reliability, which are critical for the intend longitudinal in vivo investigations. Surgically implanting the usCCW on the skull creates a self-contained environment, maintaining optical access while eliminating the need for external ultrasound coupling medium for photoacoustic imaging. Using this usCCW, we demonstrate photoacoustic microscopy of cortical vascular network in live mice over 28 days. This work establishes the foundation for integrating photoacoustic imaging with modern brain research.

Original languageEnglish (US)
Article number4277
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

Fingerprint

Lithography
lithography
Ultrasonics
Brain
brain
Photoacoustic effect
Imaging techniques
Skull
Photoacoustic microscopy
Blood Vessels
Microscopy
photoacoustic microscopy
Nanoimprint lithography
Nanophotonics
skull
mice
animals
manipulators
Research
Animals

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Disposable ultrasound-sensing chronic cranial window by soft nanoimprinting lithography. / Li, Hao; Dong, Biqin; Zhang, Xian; Shu, Xiao; Chen, Xiangfan; Hai, Rihan; Czaplewski, David A.; Zhang, Hao F.; Sun, Cheng.

In: Nature communications, Vol. 10, No. 1, 4277, 01.12.2019.

Research output: Contribution to journalArticle

Li, H, Dong, B, Zhang, X, Shu, X, Chen, X, Hai, R, Czaplewski, DA, Zhang, HF & Sun, C 2019, 'Disposable ultrasound-sensing chronic cranial window by soft nanoimprinting lithography', Nature communications, vol. 10, no. 1, 4277. https://doi.org/10.1038/s41467-019-12178-6
Li, Hao ; Dong, Biqin ; Zhang, Xian ; Shu, Xiao ; Chen, Xiangfan ; Hai, Rihan ; Czaplewski, David A. ; Zhang, Hao F. ; Sun, Cheng. / Disposable ultrasound-sensing chronic cranial window by soft nanoimprinting lithography. In: Nature communications. 2019 ; Vol. 10, No. 1.
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