Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines

George Georgiou; Christos Stathopoulos; Patrick S. Daugherty; Amiya R. Nayak; Brent L. Iverson; Roy Curtiss

doi:10.1038/nbt0197-29

Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines

George Georgiou, Christos Stathopoulos, Patrick S. Daugherty, Amiya R. Nayak, Brent L. Iverson, Roy Curtiss

Research output: Contribution to journal › Review article › peer-review

453 Scopus citations

Abstract

In recent years there has been considerable progress towards the development of expression systems for the display of heterologous polypeptides and, to a lesser extent, oligosaccharides on the surface of bacteria or yeast. The availability of protein display vectors has in turn provided the impetus for a range of exciting technologies. Polypeptide libraries can be displayed in bacteria and screened by cell sorting techniques, thus simplifying the isolation of proteins with high affinity for ligands. Expression of antigens on the surface of nonvirulent microorganisms is an attractive approach to the development of high-efficacy recombinant live vaccines. Finally, cells displaying protein receptors or antibodies are of use for analytical applications and bioseparations.

Original language	English (US)
Pages (from-to)	29-34
Number of pages	6
Journal	Nature biotechnology
Volume	15
Issue number	1
DOIs	https://doi.org/10.1038/nbt0197-29
State	Published - Jan 1997
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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AU - Iverson, Brent L.

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Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines

Abstract

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