Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)

Thomas J. Siegrist, Phillip D. Anderson, Wing H. Huen, Gregory T. Kleinheinz, Colleen M. McDermott, Todd Sandrin

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

A rapid and reliable bacterial source tracking (BST) method is essential to counter risks to human health posed by fecal contamination of surface waters. Genetic fingerprinting methods, such as repetitive sequence based-PCR (rep-PCR), have shown promise as BST tools but are time-consuming and labor-intensive. In this work, we investigate the ability of MALDI-TOF-MS to characterize and discriminate between closely related environmental strains of Escherichia coli and to classify them according to their respective sources. We compared the performance of a rapid MALDI-TOF-MS-based method to a commonly used rep-PCR-based method that employs the BOX-A1R primer. Among the criteria evaluated were repeatability and the ability of each method to group E. coli isolates according to their respective sources. Our data suggest that the MALDI-TOF-MS-based approach has a lower repeatability level compared to rep-PCR but offers an improved ability to correctly assign E. coli isolates to specific source groups. In addition, we have identified five biomarkers that appear conserved among avian species. We conclude that MALDI-TOF-MS may represent a promising, novel and rapid approach to addressing the problem of fecal contamination of surface waters and warrants further investigation.

Original languageEnglish (US)
Pages (from-to)554-562
Number of pages9
JournalJournal of Microbiological Methods
Volume68
Issue number3
DOIs
StatePublished - Mar 2007
Externally publishedYes

Fingerprint

Mass Spectrometry
Lasers
Escherichia coli
Nucleic Acid Repetitive Sequences
Polymerase Chain Reaction
DNA Fingerprinting
Water
Biomarkers
Health

Keywords

  • Bacterial source tracking (BST)
  • E. coli
  • MALDI

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). / Siegrist, Thomas J.; Anderson, Phillip D.; Huen, Wing H.; Kleinheinz, Gregory T.; McDermott, Colleen M.; Sandrin, Todd.

In: Journal of Microbiological Methods, Vol. 68, No. 3, 03.2007, p. 554-562.

Research output: Contribution to journalArticle

Siegrist, Thomas J. ; Anderson, Phillip D. ; Huen, Wing H. ; Kleinheinz, Gregory T. ; McDermott, Colleen M. ; Sandrin, Todd. / Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In: Journal of Microbiological Methods. 2007 ; Vol. 68, No. 3. pp. 554-562.
@article{2a0b520cf82d4547a0e79d1435d3b6c0,
title = "Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)",
abstract = "A rapid and reliable bacterial source tracking (BST) method is essential to counter risks to human health posed by fecal contamination of surface waters. Genetic fingerprinting methods, such as repetitive sequence based-PCR (rep-PCR), have shown promise as BST tools but are time-consuming and labor-intensive. In this work, we investigate the ability of MALDI-TOF-MS to characterize and discriminate between closely related environmental strains of Escherichia coli and to classify them according to their respective sources. We compared the performance of a rapid MALDI-TOF-MS-based method to a commonly used rep-PCR-based method that employs the BOX-A1R primer. Among the criteria evaluated were repeatability and the ability of each method to group E. coli isolates according to their respective sources. Our data suggest that the MALDI-TOF-MS-based approach has a lower repeatability level compared to rep-PCR but offers an improved ability to correctly assign E. coli isolates to specific source groups. In addition, we have identified five biomarkers that appear conserved among avian species. We conclude that MALDI-TOF-MS may represent a promising, novel and rapid approach to addressing the problem of fecal contamination of surface waters and warrants further investigation.",
keywords = "Bacterial source tracking (BST), E. coli, MALDI",
author = "Siegrist, {Thomas J.} and Anderson, {Phillip D.} and Huen, {Wing H.} and Kleinheinz, {Gregory T.} and McDermott, {Colleen M.} and Todd Sandrin",
year = "2007",
month = "3",
doi = "10.1016/j.mimet.2006.10.012",
language = "English (US)",
volume = "68",
pages = "554--562",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)

AU - Siegrist, Thomas J.

AU - Anderson, Phillip D.

AU - Huen, Wing H.

AU - Kleinheinz, Gregory T.

AU - McDermott, Colleen M.

AU - Sandrin, Todd

PY - 2007/3

Y1 - 2007/3

N2 - A rapid and reliable bacterial source tracking (BST) method is essential to counter risks to human health posed by fecal contamination of surface waters. Genetic fingerprinting methods, such as repetitive sequence based-PCR (rep-PCR), have shown promise as BST tools but are time-consuming and labor-intensive. In this work, we investigate the ability of MALDI-TOF-MS to characterize and discriminate between closely related environmental strains of Escherichia coli and to classify them according to their respective sources. We compared the performance of a rapid MALDI-TOF-MS-based method to a commonly used rep-PCR-based method that employs the BOX-A1R primer. Among the criteria evaluated were repeatability and the ability of each method to group E. coli isolates according to their respective sources. Our data suggest that the MALDI-TOF-MS-based approach has a lower repeatability level compared to rep-PCR but offers an improved ability to correctly assign E. coli isolates to specific source groups. In addition, we have identified five biomarkers that appear conserved among avian species. We conclude that MALDI-TOF-MS may represent a promising, novel and rapid approach to addressing the problem of fecal contamination of surface waters and warrants further investigation.

AB - A rapid and reliable bacterial source tracking (BST) method is essential to counter risks to human health posed by fecal contamination of surface waters. Genetic fingerprinting methods, such as repetitive sequence based-PCR (rep-PCR), have shown promise as BST tools but are time-consuming and labor-intensive. In this work, we investigate the ability of MALDI-TOF-MS to characterize and discriminate between closely related environmental strains of Escherichia coli and to classify them according to their respective sources. We compared the performance of a rapid MALDI-TOF-MS-based method to a commonly used rep-PCR-based method that employs the BOX-A1R primer. Among the criteria evaluated were repeatability and the ability of each method to group E. coli isolates according to their respective sources. Our data suggest that the MALDI-TOF-MS-based approach has a lower repeatability level compared to rep-PCR but offers an improved ability to correctly assign E. coli isolates to specific source groups. In addition, we have identified five biomarkers that appear conserved among avian species. We conclude that MALDI-TOF-MS may represent a promising, novel and rapid approach to addressing the problem of fecal contamination of surface waters and warrants further investigation.

KW - Bacterial source tracking (BST)

KW - E. coli

KW - MALDI

UR - http://www.scopus.com/inward/record.url?scp=33847419634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847419634&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2006.10.012

DO - 10.1016/j.mimet.2006.10.012

M3 - Article

VL - 68

SP - 554

EP - 562

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 3

ER -