TY - JOUR
T1 - Discovery of the first maize-infecting mastrevirus in the Americas using a vector-enabled metagenomics approach
AU - Fontenele, Rafaela S.
AU - Alves-Freitas, Dione M.T.
AU - Silva, Pedro I.T.
AU - Foresti, Josemar
AU - Silva, P. R.
AU - Godinho, Márcio T.
AU - Varsani, Arvind
AU - Ribeiro, Simone G.
N1 - Funding Information:
Acknowledgements This research was partly supported by internal Grants from Embrapa (Brazil) and a Grant (314732/2014-4) from CNPq (Brazil). RSF, DMTAF, MTG were supported by fellowships from CNPq.
Publisher Copyright:
© 2017, Springer-Verlag GmbH Austria.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The genus Mastrevirus (family Geminiviridae) is composed of single-stranded DNA viruses that infect mono- and dicotyledonous plants and are transmitted by leafhoppers. In South America, there have been only two previous reports of mastreviruses, both identified in sweet potatoes (from Peru and Uruguay). As part of a general viral surveillance program, we used a vector-enabled metagenomics (VEM) approach and sampled leafhoppers (Dalbulus maidis) in Itumbiara (State of Goiás), Brazil. High-throughput sequencing of viral DNA purified from the leafhopper sample revealed mastrevirus-like contigs. Using a set of abutting primers, a 2746-nt circular genome was recovered. The circular genome has a typical mastrevirus genome organization and shares <63% pairwise identity with other mastrevirus isolates from around the world. Therefore, the new mastrevirus was tentatively named “maize striate mosaic virus”. Seventeen maize leaf samples were collected in the same field as the leafhoppers, and ten samples were found to be positive for this mastrevirus. Furthermore, the ten genomes recovered from the maize samples share >99% pairwise identity with the one from the leafhopper. This is the first report of a maize-infecting mastrevirus in the Americas, the first identified in a non-vegetatively propagated mastrevirus host in South America, and the first mastrevirus to be identified in Brazil.
AB - The genus Mastrevirus (family Geminiviridae) is composed of single-stranded DNA viruses that infect mono- and dicotyledonous plants and are transmitted by leafhoppers. In South America, there have been only two previous reports of mastreviruses, both identified in sweet potatoes (from Peru and Uruguay). As part of a general viral surveillance program, we used a vector-enabled metagenomics (VEM) approach and sampled leafhoppers (Dalbulus maidis) in Itumbiara (State of Goiás), Brazil. High-throughput sequencing of viral DNA purified from the leafhopper sample revealed mastrevirus-like contigs. Using a set of abutting primers, a 2746-nt circular genome was recovered. The circular genome has a typical mastrevirus genome organization and shares <63% pairwise identity with other mastrevirus isolates from around the world. Therefore, the new mastrevirus was tentatively named “maize striate mosaic virus”. Seventeen maize leaf samples were collected in the same field as the leafhoppers, and ten samples were found to be positive for this mastrevirus. Furthermore, the ten genomes recovered from the maize samples share >99% pairwise identity with the one from the leafhopper. This is the first report of a maize-infecting mastrevirus in the Americas, the first identified in a non-vegetatively propagated mastrevirus host in South America, and the first mastrevirus to be identified in Brazil.
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U2 - 10.1007/s00705-017-3571-2
DO - 10.1007/s00705-017-3571-2
M3 - Article
C2 - 28956174
AN - SCOPUS:85030104946
SN - 0304-8608
VL - 163
SP - 263
EP - 267
JO - Archives of virology
JF - Archives of virology
IS - 1
ER -