Discovery of cationic polymers for non-viral gene delivery using combinatorial approaches

Sutapa Barua, James Ramos, Thrimoorthy Potta, David Taylor, Huang Chiao Huang, Gabriela Montanez, Kaushal Rege

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Gene therapy is an attractive treatment option for diseases of genetic origin, including several cancers and cardiovascular diseases. While viruses are effective vectors for delivering exogenous genes to cells, concerns related to insertional mutagenesis, immunogenicity, lack of tropism, decay and high production costs necessitate the discovery of non-viral methods. Significant efforts have been focused on cationic polymers as non-viral alternatives for gene delivery. Recent studies have employed combinatorial syntheses and parallel screening methods for enhancing the efficacy of gene delivery, biocompatibility of the delivery vehicle, and overcoming cellular level barriers as they relate to polymermediated transgene uptake, transport, transcription, and expression. This review summarizes and discusses recent advances in combinatorial syntheses and parallel screening of cationic polymer libraries for the discovery of efficient and safe gene delivery systems.

Original languageEnglish (US)
Pages (from-to)908-924
Number of pages17
JournalCombinatorial Chemistry and High Throughput Screening
Volume14
Issue number10
DOIs
StatePublished - Dec 1 2011

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Keywords

  • Cytotoxicity
  • Gene delivery
  • Parallel screening
  • Polymer library
  • Transfection
  • Transgene expression

ASJC Scopus subject areas

  • Drug Discovery
  • Computer Science Applications
  • Organic Chemistry

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