Discovery of a novel circular single-stranded DNA virus from porcine faeces

Alyssa Sikorski, Gerardo R. Argüello-Astorga, Anisha Dayaram, Renwick C J Dobson, Arvind Varsani

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A large number of novel single-stranded DNA (ssDNA) viruses have been characterised from various environmental sources in the last 5 years. The bulk of these have been from faecal sources, and faecal sampling is an ideal non-invasive pathogen sampling method. We characterised a novel ssDNA from a porcine faecal sample from Cass Basin of the South Island of New Zealand. The novel viral genome has two large open reading frames (ORFs), which are bidirectionally transcribed and separated by intergenic regions. The largest ORF has some degree of similarity (<30 %) to the putative capsid protein of chimpanzee stool-associated circular ssDNA virus (ChiSCV) and pig stool-associated single-stranded DNA virus (PigSCV), whereas the second-largest ORF has high similarity to the putative replication-associated protein (Rep) of ChiSCV (~50 %) and bovine stool-associated circular DNA virus (BoSCV; ~30 %). Based on genome architecture, location of putative stem-loop like elements, and maximum-likelihood phylogenetic analysis of the gene encoding the Rep protein, the novel isolate belongs to the same family of ssDNA viruses as ChiSCV and BoSCV.

Original languageEnglish (US)
Pages (from-to)283-289
Number of pages7
JournalArchives of Virology
Volume158
Issue number1
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Circular DNA
DNA Viruses
Single-Stranded DNA
Feces
Swine
Pan troglodytes
Open Reading Frames
Viruses
Proteins
Intergenic DNA
Viral Genome
Capsid Proteins
New Zealand
Islands
Genome
Genes

ASJC Scopus subject areas

  • Virology

Cite this

Discovery of a novel circular single-stranded DNA virus from porcine faeces. / Sikorski, Alyssa; Argüello-Astorga, Gerardo R.; Dayaram, Anisha; Dobson, Renwick C J; Varsani, Arvind.

In: Archives of Virology, Vol. 158, No. 1, 2013, p. 283-289.

Research output: Contribution to journalArticle

Sikorski, A, Argüello-Astorga, GR, Dayaram, A, Dobson, RCJ & Varsani, A 2013, 'Discovery of a novel circular single-stranded DNA virus from porcine faeces', Archives of Virology, vol. 158, no. 1, pp. 283-289. https://doi.org/10.1007/s00705-012-1470-0
Sikorski, Alyssa ; Argüello-Astorga, Gerardo R. ; Dayaram, Anisha ; Dobson, Renwick C J ; Varsani, Arvind. / Discovery of a novel circular single-stranded DNA virus from porcine faeces. In: Archives of Virology. 2013 ; Vol. 158, No. 1. pp. 283-289.
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