Discontinuities at the DNA supercoiling transition

Bryan C. Daniels, Scott Forth, Maxim Y. Sheinin, Michelle D. Wang, James P. Sethna

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

While slowly turning the ends of a single molecule of DNA at constant applied force, a discontinuity was recently observed at the supercoiling transition when a small plectoneme is suddenly formed. This can be understood as an abrupt transition into a state in which stretched and plectonemic DNA coexist. We argue that there should be discontinuities in both the extension and the torque at the transition and provide experimental evidence for both. To predict the sizes of these discontinuities and how they change with the overall length of DNA, we organize a phenomenological theory for the coexisting plectonemic state in terms of four parameters. We also test supercoiling theories, including our own elastic rod simulation, finding discrepancies with experiment that can be understood in terms of the four coexisting state parameters.

Original languageEnglish (US)
Article number040901
JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume80
Issue number4
DOIs
StatePublished - Oct 15 2009
Externally publishedYes

ASJC Scopus subject areas

  • Statistical and Nonlinear Physics
  • Statistics and Probability
  • Condensed Matter Physics

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