Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

Guoliang Ke; Minghui Liu; Shuoxing Jiang; Xiaodong Qi; Yuhe Renee Yang; Shaun Wootten; Fei Zhang; Zhi Zhu; Yan Liu; Chaoyong James Yang; Hao Yan

doi:10.1002/anie.201603183

Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

Guoliang Ke, Minghui Liu, Shuoxing Jiang, Xiaodong Qi, Yuhe Renee Yang, Shaun Wootten, Fei Zhang, Zhi Zhu, Yan Liu, Chaoyong James Yang, Hao Yan

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD⁺substrate channeling by specifically shifting NAD⁺between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.

Original language	English (US)
Pages (from-to)	7483-7486
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	55
Issue number	26
DOIs	https://doi.org/10.1002/anie.201603183
State	Published - Jun 20 2016

Keywords

DNA origami
NADH
enzymes
self-assembly
substrate channeling

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201603183

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Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold. / Ke, Guoliang; Liu, Minghui; Jiang, Shuoxing; Qi, Xiaodong; Yang, Yuhe Renee; Wootten, Shaun; Zhang, Fei; Zhu, Zhi; Liu, Yan; Yang, Chaoyong James; Yan, Hao.

In: Angewandte Chemie - International Edition, Vol. 55, No. 26, 20.06.2016, p. 7483-7486.

Research output: Contribution to journal › Article › peer-review

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title = "Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold",

abstract = "Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD+substrate channeling by specifically shifting NAD+between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.",

keywords = "DNA origami, NADH, enzymes, self-assembly, substrate channeling",

author = "Guoliang Ke and Minghui Liu and Shuoxing Jiang and Xiaodong Qi and Yang, {Yuhe Renee} and Shaun Wootten and Fei Zhang and Zhi Zhu and Yan Liu and Yang, {Chaoyong James} and Hao Yan",

note = "Funding Information: We are grateful for a DOD-Navy-ONR MURI award (W911NF-12-1-0420) and an Army Research Office grant (W911NF-11-1-0137) and thank the National Science Foundation (14030615), the National Science Foundation of China (21325522, 21422506, 21275122, 21521004), the Program for Changjiang Scholars and Innovative Research Team in University (IRT13036), and the China Scholarship Council Fellowship for financial support.",

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AU - Ke, Guoliang

AU - Liu, Minghui

AU - Jiang, Shuoxing

AU - Qi, Xiaodong

AU - Yang, Yuhe Renee

AU - Wootten, Shaun

AU - Zhang, Fei

AU - Zhu, Zhi

AU - Liu, Yan

AU - Yang, Chaoyong James

AU - Yan, Hao

N1 - Funding Information: We are grateful for a DOD-Navy-ONR MURI award (W911NF-12-1-0420) and an Army Research Office grant (W911NF-11-1-0137) and thank the National Science Foundation (14030615), the National Science Foundation of China (21325522, 21422506, 21275122, 21521004), the Program for Changjiang Scholars and Innovative Research Team in University (IRT13036), and the China Scholarship Council Fellowship for financial support.

PY - 2016/6/20

Y1 - 2016/6/20

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KW - NADH

KW - enzymes

KW - self-assembly

KW - substrate channeling

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