Abstract
Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD+substrate channeling by specifically shifting NAD+between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7483-7486 |
| Number of pages | 4 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 55 |
| Issue number | 26 |
| DOIs | |
| State | Published - Jan 1 2016 |
Keywords
- DNA origami
- NADH
- enzymes
- self-assembly
- substrate channeling
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)
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Ke, G., Liu, M., Jiang, S., Qi, X., Yang, Y. R., Wootten, S., Zhang, F., Zhu, Z., Liu, Y., Yang, C. J., & Yan, H. (2016). Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold. Angewandte Chemie - International Edition, 55(26), 7483-7486. https://doi.org/10.1002/anie.201603183