Abstract
Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD+substrate channeling by specifically shifting NAD+between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.
Original language | English (US) |
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Pages (from-to) | 7483-7486 |
Number of pages | 4 |
Journal | Angewandte Chemie - International Edition |
Volume | 55 |
Issue number | 26 |
DOIs | |
State | Published - Jun 20 2016 |
Keywords
- DNA origami
- NADH
- enzymes
- self-assembly
- substrate channeling
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)