@article{359b23af8bbc4cf1844d5a8aa8760272,
title = "Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold",
abstract = "Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD+substrate channeling by specifically shifting NAD+between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.",
keywords = "DNA origami, NADH, enzymes, self-assembly, substrate channeling",
author = "Guoliang Ke and Minghui Liu and Shuoxing Jiang and Xiaodong Qi and Yang, {Yuhe Renee} and Shaun Wootten and Fei Zhang and Zhi Zhu and Yan Liu and Yang, {Chaoyong James} and Hao Yan",
note = "Funding Information: We are grateful for a DOD-Navy-ONR MURI award (W911NF-12-1-0420) and an Army Research Office grant (W911NF-11-1-0137) and thank the National Science Foundation (14030615), the National Science Foundation of China (21325522, 21422506, 21275122, 21521004), the Program for Changjiang Scholars and Innovative Research Team in University (IRT13036), and the China Scholarship Council Fellowship for financial support. Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2016",
month = jun,
day = "20",
doi = "10.1002/anie.201603183",
language = "English (US)",
volume = "55",
pages = "7483--7486",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "26",
}