Direct phasing in femtosecond nanocrystallography. II. Phase retrieval

Joe P J Chen, John Spence, Rick P. Millane

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    X-ray free-electron laser diffraction patterns from protein nanocrystals provide information on the diffracted amplitudes between the Bragg reflections, offering the possibility of direct phase retrieval without the use of ancillary experimental diffraction data [Spence et al. (2011). Opt. Express, 19, 2866-2873]. The estimated continuous transform is highly noisy however [Chen et al. (2014). Acta Cryst. A70, 143-153]. This second of a series of two papers describes a data-selection strategy to ameliorate the effects of the high noise levels and the subsequent use of iterative phase-retrieval algorithms to reconstruct the electron density. Simulation results show that employing such a strategy increases the noise levels that can be tolerated.

    Original languageEnglish (US)
    Pages (from-to)154-161
    Number of pages8
    JournalActa Crystallographica Section A: Foundations and Advances
    Volume70
    Issue number2
    DOIs
    StatePublished - 2014

    Fingerprint

    X ray lasers
    Free electron lasers
    Nanocrystals
    Diffraction patterns
    retrieval
    Carrier concentration
    Noise
    Diffraction
    Electrons
    Proteins
    free electron lasers
    Nanoparticles
    nanocrystals
    Lasers
    diffraction patterns
    X-Rays
    proteins
    diffraction
    x rays
    simulation

    Keywords

    • Direct phasing
    • Femtosecond crystallography
    • Iterative transform algorithms
    • Nanocrystals
    • Shape transform
    • X-ray free-electron lasers

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry
    • Inorganic Chemistry
    • Physical and Theoretical Chemistry
    • Materials Science(all)
    • Condensed Matter Physics

    Cite this

    Direct phasing in femtosecond nanocrystallography. II. Phase retrieval. / Chen, Joe P J; Spence, John; Millane, Rick P.

    In: Acta Crystallographica Section A: Foundations and Advances, Vol. 70, No. 2, 2014, p. 154-161.

    Research output: Contribution to journalArticle

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