Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles

Jia Fan, Jun Jie Yin, Bo Ning, Xiaochun Wu, Ye Hu, Mauro Ferrari, Gregory J. Anderson, Jingyan Wei, Yuliang Zhao, Guangjun Nie

Research output: Contribution to journalArticle

207 Citations (Scopus)

Abstract

Using apoferritin (apoFt) as a nucleation substrate, we have successfully synthesized 1-2 nm platinum nanoparticles (Pt-Ft) which are highly stable. By directly measuring the products of Pt-Ft-catalyzed reactions, we showed, with no doubt, Pt-Ft possesses both catalase and peroxidase activities. With hydrogen peroxide as substrate, we observed oxygen gas bubbles were generated from hydrogen peroxide decomposed by Pt-Ft; the generation of oxygen gas strongly supports Pt-Ft reacts as catalase, other than peroxidase. While with organic dyes and hydrogen peroxide as substrates, distinctive color products were formed catalyzed by Pt-Ft, which indicates a peroxidase-like activity. Interestingly, these biomimetic properties showed differential response to pH and temperature for different reaction substrates. Pt-Ft showed a significant increase in catalase activity with increasing pH and temperature. The HRP-like activity of Pt-Ft was optimal at physiological temperature and slightly acidic conditions. Our current study demonstrates that Pt-Ft possesses both catalase and peroxidase activities for different substrates under different conditions.

Original languageEnglish (US)
Pages (from-to)1611-1618
Number of pages8
JournalBiomaterials
Volume32
Issue number6
DOIs
StatePublished - Feb 1 2011
Externally publishedYes

Fingerprint

Ferritins
Platinum
Nanoparticles
Catalase
Peroxidase
Hydrogen Peroxide
Hydrogen peroxide
Substrates
Temperature
Gases
Apoferritins
Oxygen
Biomimetics
Coloring Agents
Color
Nucleation
Thermodynamic properties
Dyes

Keywords

  • Biomimetic enzymes
  • Catalase activities
  • ESR
  • Ferritin
  • Nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Fan, J., Yin, J. J., Ning, B., Wu, X., Hu, Y., Ferrari, M., ... Nie, G. (2011). Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles. Biomaterials, 32(6), 1611-1618. https://doi.org/10.1016/j.biomaterials.2010.11.004

Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles. / Fan, Jia; Yin, Jun Jie; Ning, Bo; Wu, Xiaochun; Hu, Ye; Ferrari, Mauro; Anderson, Gregory J.; Wei, Jingyan; Zhao, Yuliang; Nie, Guangjun.

In: Biomaterials, Vol. 32, No. 6, 01.02.2011, p. 1611-1618.

Research output: Contribution to journalArticle

Fan, J, Yin, JJ, Ning, B, Wu, X, Hu, Y, Ferrari, M, Anderson, GJ, Wei, J, Zhao, Y & Nie, G 2011, 'Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles', Biomaterials, vol. 32, no. 6, pp. 1611-1618. https://doi.org/10.1016/j.biomaterials.2010.11.004
Fan, Jia ; Yin, Jun Jie ; Ning, Bo ; Wu, Xiaochun ; Hu, Ye ; Ferrari, Mauro ; Anderson, Gregory J. ; Wei, Jingyan ; Zhao, Yuliang ; Nie, Guangjun. / Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles. In: Biomaterials. 2011 ; Vol. 32, No. 6. pp. 1611-1618.
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