Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1 × 10-8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator-free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.
ASJC Scopus subject areas
- Analytical Chemistry