Direct electrochemical monitoring of RNase activity

Yongkang Ye; Wen Wen; Yun Xiang; Xiaodong Qi; Jeffrey LaBelle; Julian Chen; Joseph Wang

doi:10.1002/elan.200804172

Direct electrochemical monitoring of RNase activity

Yongkang Ye, Wen Wen, Yun Xiang, Xiaodong Qi, Jeffrey LaBelle, Julian Chen, Joseph Wang

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1 × 10^-8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator-free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.

Original language	English (US)
Pages (from-to)	919-922
Number of pages	4
Journal	Electroanalysis
Volume	20
Issue number	8
DOIs	https://doi.org/10.1002/elan.200804172
State	Published - Apr 2008

Keywords

Electrochemistry
Guanine
Ribonuclease

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.200804172

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abstract = "Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1 × 10-8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator-free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.",

keywords = "Electrochemistry, Guanine, Ribonuclease",

author = "Yongkang Ye and Wen Wen and Yun Xiang and Xiaodong Qi and Jeffrey LaBelle and Julian Chen and Joseph Wang",

year = "2008",

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T1 - Direct electrochemical monitoring of RNase activity

AU - Ye, Yongkang

AU - Wen, Wen

AU - Xiang, Yun

AU - Qi, Xiaodong

AU - LaBelle, Jeffrey

AU - Chen, Julian

AU - Wang, Joseph

PY - 2008/4

Y1 - 2008/4

N2 - Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1 × 10-8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator-free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.

AB - Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1 × 10-8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator-free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.

KW - Electrochemistry

KW - Guanine

KW - Ribonuclease

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M3 - Article

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VL - 20

SP - 919

EP - 922

JO - Electroanalysis

JF - Electroanalysis

IS - 8

ER -

Direct electrochemical monitoring of RNase activity

Abstract

Keywords

ASJC Scopus subject areas

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