Direct electrochemical monitoring of RNase activity

Yongkang Ye, Wen Wen, Yun Xiang, Xiaodong Qi, Jeffrey LaBelle, Julian Chen, Joseph Wang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1 × 10-8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator-free electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.

Original languageEnglish (US)
Pages (from-to)919-922
Number of pages4
JournalElectroanalysis
Volume20
Issue number8
DOIs
StatePublished - Apr 1 2008

Keywords

  • Electrochemistry
  • Guanine
  • Ribonuclease

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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