Direct detection of peptides and proteins on a microfluidic platform with MALDI mass spectrometry

Mian Yang, Tzu Chiao Chao, Randall Nelson, Alexandra Ros

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The ability to detect and quantify proteins of individual cells in high throughput is of enormous biological and clinical relevance. Most methods currently in use either require the measurement of large cell populations or are limited to the investigation of few cells at a time. In this report, we present the combination of a polydimethylsiloxane-based microfluidic device to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) that allows the detection of as few as 300 molecules at the peptide level and ~106 to 107 molecules at the protein level. Moreover, we performed an immunoassay with subsequent MALDI-TOF-MS to capture and detect insulin immobilized on a surface (~0.05 mm2) in this device with a detection limit of 106 insulin molecules. This microfluidic-based approach therefore begins to approach the sample handling and sensitivity requirements for MS-based single-cell analysis of proteins and peptides and holds the potential for easy parallelization of immunoassays and other highly sensitive protein analyses.

Original languageEnglish (US)
Pages (from-to)1681-1689
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume404
Issue number6-7
DOIs
StatePublished - Oct 2012

Fingerprint

Microfluidics
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass spectrometry
Mass Spectrometry
Mass spectrometers
Lab-On-A-Chip Devices
Immunoassay
Peptides
Molecules
Ionization
Desorption
Lasers
Insulin
Single-Cell Analysis
Proteins
Limit of Detection
Cells
Throughput
Equipment and Supplies
Population

Keywords

  • Immunoassay
  • Mass spectrometry
  • Microfluidic
  • Single-cell sensitivity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Direct detection of peptides and proteins on a microfluidic platform with MALDI mass spectrometry. / Yang, Mian; Chao, Tzu Chiao; Nelson, Randall; Ros, Alexandra.

In: Analytical and Bioanalytical Chemistry, Vol. 404, No. 6-7, 10.2012, p. 1681-1689.

Research output: Contribution to journalArticle

@article{e611399918d34042ba5c6520c7594e93,
title = "Direct detection of peptides and proteins on a microfluidic platform with MALDI mass spectrometry",
abstract = "The ability to detect and quantify proteins of individual cells in high throughput is of enormous biological and clinical relevance. Most methods currently in use either require the measurement of large cell populations or are limited to the investigation of few cells at a time. In this report, we present the combination of a polydimethylsiloxane-based microfluidic device to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) that allows the detection of as few as 300 molecules at the peptide level and ~106 to 107 molecules at the protein level. Moreover, we performed an immunoassay with subsequent MALDI-TOF-MS to capture and detect insulin immobilized on a surface (~0.05 mm2) in this device with a detection limit of 106 insulin molecules. This microfluidic-based approach therefore begins to approach the sample handling and sensitivity requirements for MS-based single-cell analysis of proteins and peptides and holds the potential for easy parallelization of immunoassays and other highly sensitive protein analyses.",
keywords = "Immunoassay, Mass spectrometry, Microfluidic, Single-cell sensitivity",
author = "Mian Yang and Chao, {Tzu Chiao} and Randall Nelson and Alexandra Ros",
year = "2012",
month = "10",
doi = "10.1007/s00216-012-6257-3",
language = "English (US)",
volume = "404",
pages = "1681--1689",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "6-7",

}

TY - JOUR

T1 - Direct detection of peptides and proteins on a microfluidic platform with MALDI mass spectrometry

AU - Yang, Mian

AU - Chao, Tzu Chiao

AU - Nelson, Randall

AU - Ros, Alexandra

PY - 2012/10

Y1 - 2012/10

N2 - The ability to detect and quantify proteins of individual cells in high throughput is of enormous biological and clinical relevance. Most methods currently in use either require the measurement of large cell populations or are limited to the investigation of few cells at a time. In this report, we present the combination of a polydimethylsiloxane-based microfluidic device to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) that allows the detection of as few as 300 molecules at the peptide level and ~106 to 107 molecules at the protein level. Moreover, we performed an immunoassay with subsequent MALDI-TOF-MS to capture and detect insulin immobilized on a surface (~0.05 mm2) in this device with a detection limit of 106 insulin molecules. This microfluidic-based approach therefore begins to approach the sample handling and sensitivity requirements for MS-based single-cell analysis of proteins and peptides and holds the potential for easy parallelization of immunoassays and other highly sensitive protein analyses.

AB - The ability to detect and quantify proteins of individual cells in high throughput is of enormous biological and clinical relevance. Most methods currently in use either require the measurement of large cell populations or are limited to the investigation of few cells at a time. In this report, we present the combination of a polydimethylsiloxane-based microfluidic device to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) that allows the detection of as few as 300 molecules at the peptide level and ~106 to 107 molecules at the protein level. Moreover, we performed an immunoassay with subsequent MALDI-TOF-MS to capture and detect insulin immobilized on a surface (~0.05 mm2) in this device with a detection limit of 106 insulin molecules. This microfluidic-based approach therefore begins to approach the sample handling and sensitivity requirements for MS-based single-cell analysis of proteins and peptides and holds the potential for easy parallelization of immunoassays and other highly sensitive protein analyses.

KW - Immunoassay

KW - Mass spectrometry

KW - Microfluidic

KW - Single-cell sensitivity

UR - http://www.scopus.com/inward/record.url?scp=84867330690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867330690&partnerID=8YFLogxK

U2 - 10.1007/s00216-012-6257-3

DO - 10.1007/s00216-012-6257-3

M3 - Article

VL - 404

SP - 1681

EP - 1689

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 6-7

ER -