Direct detection of peptides and proteins on a microfluidic platform with MALDI mass spectrometry

Mian Yang, Tzu Chiao Chao, Randall Nelson, Alexandra Ros

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The ability to detect and quantify proteins of individual cells in high throughput is of enormous biological and clinical relevance. Most methods currently in use either require the measurement of large cell populations or are limited to the investigation of few cells at a time. In this report, we present the combination of a polydimethylsiloxane-based microfluidic device to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) that allows the detection of as few as 300 molecules at the peptide level and ~106 to 107 molecules at the protein level. Moreover, we performed an immunoassay with subsequent MALDI-TOF-MS to capture and detect insulin immobilized on a surface (~0.05 mm2) in this device with a detection limit of 106 insulin molecules. This microfluidic-based approach therefore begins to approach the sample handling and sensitivity requirements for MS-based single-cell analysis of proteins and peptides and holds the potential for easy parallelization of immunoassays and other highly sensitive protein analyses.

Original languageEnglish (US)
Pages (from-to)1681-1689
Number of pages9
JournalAnalytical and bioanalytical chemistry
Volume404
Issue number6-7
DOIs
StatePublished - Oct 1 2012

Keywords

  • Immunoassay
  • Mass spectrometry
  • Microfluidic
  • Single-cell sensitivity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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