Direct atomic force microscopy observation of DNA tile crystal growth at the single-molecule level

Constantine G. Evans, Rizal Hariadi, Erik Winfree

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

While the theoretical implications of models of DNA tile self-assembly have been extensively researched and such models have been used to design DNA tile systems for use in experiments, there has been little research testing the fundamental assumptions of those models. In this paper, we use direct observation of individual tile attachments and detachments of two DNA tile systems on a mica surface imaged with an atomic force microscope (AFM) to compile statistics of tile attachments and detachments. We show that these statistics fit the widely used kinetic Tile Assembly Model and demonstrate AFM movies as a viable technique for directly investigating DNA tile systems during growth rather than after assembly.

Original languageEnglish (US)
Pages (from-to)10485-10492
Number of pages8
JournalJournal of the American Chemical Society
Volume134
Issue number25
DOIs
StatePublished - Jun 27 2012
Externally publishedYes

Fingerprint

Atomic Force Microscopy
Tile
Crystallization
Crystal growth
Atomic force microscopy
DNA
Observation
Molecules
Motion Pictures
Microscopes
Statistics
Theoretical Models
Mica
Self assembly
Growth
Research
Kinetics
Testing

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Direct atomic force microscopy observation of DNA tile crystal growth at the single-molecule level. / Evans, Constantine G.; Hariadi, Rizal; Winfree, Erik.

In: Journal of the American Chemical Society, Vol. 134, No. 25, 27.06.2012, p. 10485-10492.

Research output: Contribution to journalArticle

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